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[] Oxidative potential of selected PM components

Sapienza University of Rome, Department of Chemistry, Rome, Italy
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17 July 2017
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Abstract

Particulate matter (PM) is a complex mixture of suspended solid and liquid particles characterized by toxicological properties (1). Oxidative stress is considered as one of the most important mechanism of PM toxicity on living organisms. Reactive oxygen species (ROS) may in fact damage lipids in biological membranes, enzymatic proteins and DNA. ROS content in PM samples is frequently evaluated in the literature in terms of oxidative potential (OP). However, the relations between the obtained results and the chemical and physical characteristic of particles are still largely unknown.In this work we report the results obtained by three different assays for measuring oxidative potential (OP) on selected components of PM having a very different chemical composition (road dust, soil dust, brake dust, desert dust, pellet ash and coke and certified material NIST1648a – urban dust). The first two methods are based on the decrease of reducing species concentration (respectively Dithiothreitol - DTT and Ascorbic Acid - AA) due to ROS (2); the third one is based on the formation of a fluorescent compound (dichlorofluorescein - DCF) from oxidation of 2′,7′-dichlorofluorescein (DCFH ) in the presence of horseradish peroxidase (HRP) (3). The results show that the three methods respond very differently to each dust and that, accordingly to the results of a previous study of the oxidative stress on simple in vivo organism model (4), the un-soluble chemical components of dusts have an OP much higher than that of the soluble species.

1-Canepari et al. 2013, Aerosol and Air Quality Research 13, 1619-1629.

2-Fang et al. 2015, Atmospheric Chemistry and Physics 15, 30609–30644.

3-Huang et al. 2016, Water, Air, & Soil Pollution 227-164.

4-Marcoccia et al. 2017, Chemosphere 173, 124-134.

Keywords

particulate matter, potential oxidative, reactive oxygen species

Cite this article as

Simonetti, G.; Conte, E.; Frasca, D.; Canepari, S. Oxidative potential of selected PM components. In Proceedings of the 2nd Int. Electron. Conf. Atmos. Sci., 16–31 July 2017; Sciforum Electronic Conference Series, Vol. 2, 2017 ; doi:10.3390/ecas2017-04131

Copyright & Licensing

Copyright by the author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

Author biographies

Giulia Simonetti
Born on 22 April 1989 in Rome. In 2008 she gained her diploma at the high school “Gregorio da Catino” in Passo Corese. In 2014 she got her bachelor’s degree in analytical chemistry at Sapienza University in Rome. Her experimental degree tesi : “Particle Into Liquid Sampler for the high temporal resolution sampling and analysis of elements in Particulate matter (PM) in order to discover outdoor and indoor sources. Final grades 110/110 with honors. Since 2014 she has been working on a doctorate at the chemistry department of the Sapienza University, under the supervision of Dr. Silvia Canepari.
Daniele Frasca
Postoctoral Fellow: "Assesment of air quality in industrial areas and source apportionment". 02/2017 Doctoral degree in Infectius Diseases, Microbiology and Public Health ,thesis "Assesment of air quality in areas with different anthropic impact, the role of particulate matter and its chemical composition". 01/2013 Master's degree Environmental Monitoring and Riqualification (LM-75), 110/110 cum laude. 01/2011 Bachelor's degree in Environmental Science.
Silvia Canepari
Graduated cum laude in Chemistry in 1986, she obtained the Ph.D. in Chemistry Sciences in 1992. From 1987 to 1989 she collaborated with the Institute of Atmospheric Pollution of the National Research Council of Italy on projects about the development of an environmental sampler and the optimization of analytical procedures for the determination of organic exhaust in emissions and indoor environments. From 1989 to 2001 she was technician at Sapienza university of Rome, Centro Interdipartimentale per le Malattie Sociali - Sezione di Chimica Bioanalitica. Since 2001 she is researcher at the Department of Chemistry (Sapienza University of Rome).

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