Metabolomic to target insecticidal compounds of Caesalpinia pluviosa var. peltophoroides against Spodoptera frugiperda

¹ Laboratório de Fitoquímica e Química Medicinal - LFQM, Chemistry Institute, Universidade Federal de Alfenas - UNIFAL-MG.
² Departamento de Ciências Exatas, Universidade Federal de São Carlos – UFSCar
³ Departamento de Ciências Exatas e da Terra, Universidade Federal de São Paulo – UNIFESP

Published: 01 November 2016 by MDPI in The 1st International Electronic Conference on Metabolomics session Applications of Metabolomics in Agriculture and Industry

https://doi.org/10.3390/iecm-1-B004

Abstract:

Introduction

Spodoptera frugiperda (Lepidoptera: Noctuidae) is one of the main plagues of maize cultivation and it is resistant to the most used insecticides. Plants are important source of biopesticides due to their own mechanisms of interaction and diversified composition. Thus, fractions (hexane, acetate and hydro-ethanol ) of extracts from different parts of Caesalpinia pluviosa var. peltophoroides (Fabaceae) have insecticidal activity investigated. Chemical composition of the fractions were evaluated by UHPLC-HRFTMS, thereafter processed on MZmine 2.2 software and exported to do multivariate statistical analysis (MSA) - O2PLS-DA (SIMCA-P 13.0). The dereplication of compounds were performed using DNP^© , Scifinder^® and our in house Caesalpinia database.

Results and Discussion

Six fractions were inactive and five displayed pupal or larval mortality. The O2PLS-DA analysis found a clear separation of inactive and insecticidal group (R²= 0,72) and indicated the metabolites highly correlated with the insecticidal property (VIP>1.95): eugenyl vicianoside; gluconic acid lactone and gallic acid.

Conclusions

Thus, through metabolomic strategies was possible to dereplicate the compounds most correlated with insecticidal property and this knowledge can be very useful to development of new techniques to the crop pest control.

Acknowledgements

FAPEMIG and CNPQ for the financial funding and the AsterBioChem for the UHPLC-HRMS analysis.

Keywords: metabolomic, Caesalpinia pluviosa, Spodoptera frugiperda, insecticidal, UHPLC-HRMS

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