RAMAN ACTIVATED DROPLET SORTING (RADS): HIGH-THROUGHPUT SCREENING OF AXT-HIGH-YIELD HAEMATOCOCCUS PLUVIALIS MUTANTS

Xixian Wang; Bo Ma

doi:10.3390/optofluidics2017-04306

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RAMAN ACTIVATED DROPLET SORTING (RADS): HIGH-THROUGHPUT SCREENING OF AXT-HIGH-YIELD HAEMATOCOCCUS PLUVIALIS MUTANTS

Xixian Wang

Bo Ma

¹ Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences,

Published: 21 July 2017 by MDPI in The 7th International Multidisciplinary Conference on Optofluidics 2017 session Droplets and emulsions

https://doi.org/10.3390/optofluidics2017-04306

Abstract:

This paper presents a high -throughput and -efficiency microfluidic Raman-activated droplet sorting (RADS) system combining many of the advantages of microtitre-plate screening. To validate the RADS system, the astaxanthin (AXT)-high-yield Haematococcus pluvialis (H. pluvialis) cells were sorted from the mixtures of cells, accumulating different amount of AXT.

Keywords: microdroplet, Raman spectroscopy, single-cell sorting, microalgae

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Xixian Wang

Bo Ma