Qualitative phenolic profile of Rumex tunetanus flowers and stems using ultra-high-performance liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry and their antioxidant activity

: The present study was designed to investigate the bioactive compound in Rumextunetanus extracts (polygonaceae), a plant growing in GarâaSejnane region (NW-Tunisia). Hydro-methanol extracts of flowers and stems of Rumextunetanus were analyzed by RP-UHPLC-ESI-QTOF-MS in the negative mode to identify the maximum of bioactive compounds. Applied the aforementioned method, a total of 60 bioactive compounds were characterized for the first time in Rumextunetanus between them, 18 photochemical were firstly identified in the Polygonaceae family in negative ionization mode. Quantification of the identified compounds revealed that quercetin-3-O-glucuronide and (-)-epicatechingallate were the most abundant phenolic compounds in flowers and stems, respectively. Moreover, positive correlations were found between the antioxidant activity measured by DPPH and FRAP assays with the total phenolic compounds (r = 0.98; r = 0.99, respectively) and the abundance of some phenolic subfamilies such as hydroxycinnamic acids, hydroxybenzoic acids, flavonols and flavones with r > 0.86. The compounds displaying significant (P < 0.01) and good correlations with the antioxidant activity (r > 0.93) were hydroxybenzoic acid, rutin, quercetin-3-O-glucuronide, quercetin-3-O-glucoside, quercetin and luteolin-7-O-rutinoside. In addition, the flowers and stems of Rumex tunetanus showed different bioactive compound profiles and significant antioxidant properties of extracts. These results highlight the potential of the RP-UHPLC-ESI-QTOF-MS and MS/MS system to identify untargeted metabolic profiling of Rumex tunetanus . Overall, these results contribute to the clear explanation of the past and current usage of genus Rumex in folk medicine. Future investigations are necessary to develop purified antioxidant extracts, with the application of more selective extraction techniques.


Introduction
In response to the increased popularity and greater demand for medicinal plants, a recent study by world health organization claimed 80% dependency of word population on ethnomedicines [1].
In fact, the importance of medicinal plants in solving the health care problems of the world is gaining increasing attention and it is growing phenomenally at the international level.
However, the pharmacological evaluation of substances from plants is an established method for the identification of new compounds which can leads to the development of novel and safe medicinal agents in order to increase the dangerous side effects of synthetic molecules

Total phenolic content (TPC) and antioxidant activity of the Rumex tunetanus flowers and stems
The determination of TPC of the extracts was performed by the Folin-Ciocalteu assay and calculated as gallic acid equivalents and is listed in Table 1.Rumex tunetanus flowers extract was found to contain higher total phenolic contents (146.20 mg GAE/g extract) as compared to Rumex tunetanus stems extract (118.88 mg GAE/g extract).
The extracts were evaluated for their antioxidant /radical scavenging activity by DPPH and FRAP.The results of percentage scavenging are depicted in Table 1.In general, both of these two analyses showed that the hydro-methanolic extract of Rumex tunetanus exhibited good antioxidant activities.According to the aforementioned results for TPC, the flowers showed higher antioxidant activity values than stems by the two assayed methods.
In fact, our results have indicated that the DPPH value of Rumex tunetanus flowers and stems extracts are in accordance with those obtained by Yousef et al. [11] forRumex tuberosus flowers (0,65mg/ml of extract) and stems (0,69 mg/ml of extract).

Characterization strategy and fragmentation pattern study
The

Comparison between flowers and stems
Table 1 shows the qualitative differences

Solvents and standards
The solvents used for extraction were ultrapure Flowers and stems were extracted using methanol/water 80:20 (v/v) as described elsewhere [7].The residue were filtered with a syringe filter (regenerated cellulose, 0.45μm pore size) and stored at −20 °C until future analysis.
The extractions were repeated twice for each studied rumex tunetanus part.

Total phenol content and antioxidant capacity assays
The TPC of the extracts was determined in triplicate by the colorimetric assay using the Folin-Ciocalteau reagent as reported by Ammar et al. [8].The DPPH assay was based on the method described by Gargouri et al. [9].The FRAP assay was conducted following the method described by Ammar et al. [10].

[ 2 ]
.The genus Rumex represents one of the most important genera of Polygonaceae family, including approximately 200 species widely distributed in North American, European, African and Ausian countries [3].Since very old times, differents species of rumex genus have been used for relief of symptoms of diseases.Much interest in Rumex species emanates from their long use in folk medicines as well as their pharmacological properties pharmacological activities such as anti-inflammatory, antidiuretic, antitumor, analgesic, antifungal and anti-viral activities [1].Large number of medicinal plants have been investigated for their antioxidant properties .Polyphenols are considered among the most important antioxidants in humain diet, and their presence in plant can protect consumers against oxidative stress , cardiovascular and chronic diseases [4].In fact, Rumex genus emerged as a good source of natural antioxidants.The major types of phenolic antioxidants found in Rumex genus include phenolic acids and their derivatives, namely gallic acid, dihydroxybenzoic acid, hydroxybenzoic acid and vanillic acid, and flavonoids, namely flavan-3-ols(catechin, epicatechin and epicatechin gallate), flavonols (rutin, quercetin-3-O-glucoside, quercetin-3-Oglucuronide, quercetin and isoramnetin), as well as condensed tannins as B-type procyanidin dimer and A-type procyanidin trimer[5].Tunisia flora is known for its diversity of medicinal plant among them Rumex tunetanus.This plant was growing in the wet marshes of the Sejenane plain.Rumex tunetanus was never seen after its discovery in 1888, and more than 120 years after the discovery of this rumex, we find a large population of Rumex tunetanus in december 2009[6].Therefore, as potential bioactive markers, the total phenolic content (TPC) and antioxidant capacity of flowers and stems of rumex tunetanus were firstly evaluated.Secondly, their phenolic profiles were extensively studied by ultra-high performance liquid chromatography (UHPLC) coupled with two detection systems, DAD and quadrupole time-of-flight (QTOF)-MS using electrospray ionization in negative ionization mode.
metabolic profiling of the aqueousmethanolic extracts of flowers and stems was performed by using RP-HPLC-DAD-QTOF-MS using ESI in the negative mode.This is the commonest ionization source and mode used to identify the phenolic and non-phenolic compounds in Rumex tunetanus flowers and stems.Using this methodology, our characterization steps could be basically summarized by a targeted searching of previously, an untargeted analysis and a predictive study of unreported phenolic structures based on all the spectrometric data obtained by the detection techniques applied (Figure 1).Thanks to the methodology proposed in the present study, a total of 60 metabolites (60 in flowers and 51 in stems ( between flowers and stems: presence (+) or absence (-) of the characterized compounds.In brief, more than 90% of the compounds were in common in both extracts.Moreover, as summary Figure 2 depicts these differences in terms of number of compounds found in each phenolic class.Interestingly, flavonoids and their glycoside derivatives were characterized in the negative ionization mode as a more widely spread phenolic group of Rumex tunetanus samples with 28 compounds.To the best of our knowledge, this is the first time that dihydroxybenzoic acid hexoside (8), syringic acid hexoside (12) and tri-O-methoxyellagic Mol2Net, 2018, 1(Section A, B, C, etc.), 1-x, type of paper, doi: xxx-xxxx 4 acid (51) have been identified in Polygonaceae family.Mol2Net-04, 2018, BIOCHEMPHYS-01 (pages 1-x, type of paper, doi: xxx-xxxx http://sciforum.net/conference/mol2net-4 water and methanol.All solvents used for HPLC-MS analysis were delivered by J.T Baker (Phillipsburg -USA).The reagents used to measure the TPC (total phenol content) and the antioxidant capacity were: Folin-Ciocalteau; sodium carbonate (Na2CO3); 2,2-diphenyl ,1picrylhydrazyl (DPPH); 2,4,6-tri(2-pyridyl)-1,3,5-triazine (TPTZ) and ferric chloride (FeCl3.6H2O);gallic acid, acetic acid (C2H4O2); Ferric sulfate (FeSO4); hydrochloric acid (HCl); trihydrate sodium acetate (C2H3NaO2 .3H2O);sodium acetate; ascorbic acid.The phenolic standards used in our work were bought from Sigma-Aldrich (Saint-Louis -Missouri).The degree of purity of the standards was around 95% (w/w).Plant samples procurement and extractionFlowers, stems and leaves from the Rumex tunetanus were collected from the Garâa Sejenane region (NW-Tunisia) in May 2014.The samples was harvested and transferred to the laboratory where they dried in the dark at room temperature 30 °C, and then they were finely ground prior to extraction.

Table 2
well as tannins (19).These results exemplify that RP-HPLC-DAD-QTOF-MS is useful to detect and characterize novel chemical structures.

Table 1Total phenolic content (TPC) (mg of GAE/g of the extract) determination and antioxidant capacities of the studied Rumex tunetanusflowers and stems parts as measured by DPPH (mg/ml of extract) and FRAP (mmol eq. FeSO4/ g of the extract) antioxidant assays
Figure 1: The strategy for deducing of the most possible structures of Rumex tunetanus bioactive compounds

Literature concerning Rumex Tunetanus Previously known compounds in Rumex species Targeted searching by EIC UV MS MS/MS Untargeted searching UV MS MS/MS Databases & literature Unknown compounds, but previously described in polygonaceae family Unknown compounds not reported in plants Untargeted searching Confirmation with standards Unknown compounds, but previously described in other plantsTable 2 .
Compounds characterized by HPLC-DAD-QTOF-MS in Rumex tunetanus flowers and stems * Identification confirmed by comparison with standards