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Microwave-Assisted Extraction of Herbacetin Diglucoside from Flax (Linum usitatissimum L.) Seed Cakes and Its Quantification using an RP-HPLC-UV System
Ophélie Fliniaux 1 , Cyrielle Corbin 2 , Aina Ramsay 1 , Sullivan Renouard 1 , Vickram Beejmohun 1 , Joël Doussot 2 , Annie Falguieres 3 , Clotilde Ferroud 3 , Frédéric Lamblin 2 , Eric Lainé 2 , Albrecht Roscher 4 , Eric Grand 5 , François Mesnard 1 , Christophe Hano 2
1  Laboratoire de Phytotechnologie EA 3900 BIOPI, Faculté de Pharmacie, Université de Picardie Jules Verne, 1 rue des Louvels, 80037 Amiens, France; s:(O.F.);(A.R.);(S.R.);(V.B.)
2  Laboratoire de Biologie des Ligneux et des Grandes Cultures UPRES EA 1207, Equipe Lignanes des Linacées, Université d’Orléans — Antenne Scientifique Universitaire de Chartres, 21 rue de Loigny la Bataille, 28000 Chartres, France; s:(C.C.);(J.D.);(F.L.);(E.L.);(C.H.)
3  Service de Transformations Chimiques et Pharmaceutiques, ERL CNRS 3193, Conservatoire National des Arts et Métiers, 2 rue Conté, 75003 Paris, France; s:(A.F.);(C.F.)
4  Génie Enzymatique et Cellulaire, FRE 3580 CNRS, Université de Picardie Jules Verne, 33 rue Saint-Leu, 80039 Amiens, France
5  Laboratoire des Glucides FRE 3517 CNRS, Faculté des Sciences, Université de Picardie Jules Verne, 33 rue Saint-Leu, 80039 Amiens, France

Published: 10 March 2014 by MDPI in Molecules
MDPI, Volume 19; 10.3390/molecules19033025
Abstract: Flax (Linum usitatissimum L.) seeds are widely used for oil extraction and the cold-pressed flaxseed (or linseed) cakes obtained during this process constitute a valuable by-product. The flavonol herbacetin diglucoside (HDG) has been previously reported as a constituent of the flaxseed lignan macromolecule linked through ester bonds to the linker molecule hydroxymethylglutaric acid. In this context, the development and validation of a new approach using microwave-assisted extraction (MAE) of HDG from flaxseed cakes followed by quantification with a reverse-phase HPLC system with UV detection was purposed. The experimental parameters affecting the HDG extraction yield, such as microwave power, extraction time and sodium hydroxide concentration, from the lignan macromolecule were optimized. A maximum HDG concentration of 5.76 mg/g DW in flaxseed cakes was measured following an irradiation time of 6 min, for a microwave power of 150 W using a direct extraction in 0.1 M NaOH in 70% (v/v) aqueous methanol. The optimized method was proven to be rapid and reliable in terms of precision, repeatability, stability and accuracy for the extraction of HDG. Comparison with a conventional extraction method demonstrated that MAE is more effective and less time-consuming.
Keywords: Herbacetin Diglucoside, microwave-assisted extraction, Flaxseed, flavonol, Linum usitatissimum
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