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Two complete chloroplast genome sequences and intra-species diversity for Rehmannia glutinosa (Orobanchaceae)
Jae-Hyeon Jeon, 1 Hyun-Seung Park, 1 Jee Young Park, 1 Tae Sun Kang, 2 Kisung Kwon, 2 Yeon Bok Kim, 3 Jong-Won Han, 4 Seung Hyun Kim, 5 Sang Hyun Sung, 6 Tae-Jin Yang 1
1  Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea
2  New Hazardous Substances Team, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Ohsong, Republic of Korea
3  Department of Medicinal & Industrial Crops, Korea National College of Agriculture and Fisheries, Jeonju, Republic of Korea
4  Herbal Crop Research Division, Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Eumseong, Republic of Korea
5  College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon, Republic of Korea
6  College of Pharmacy, Research Institute of Pharmaceutical Science, Seoul National University, Seoul, Republic of Korea

Published: 02 January 2019 by Informa UK Limited in Mitochondrial DNA Part B
Informa UK Limited, Volume 4; 10.1080/23802359.2018.1545529
Abstract: Rehmannia glutinosa is a plant used as traditional medicine for its various tonic effects in Korea and China. In this study, chloroplast genomes of two R. glutinosa were completed by de novo assembly using whole-genome Illumina sequence data. The length of chloroplast genomes of R. glutinosa collected from China and Korea was 153,680 bp and 153,499 bp, respectively. A total of 114 coding regions were predicted in both R. glutinosa including 80 protein-coding genes, 4 rRNA genes, and 30 tRNA genes. We identified abundant intra-species diversity of 87 InDels and 147 SNPs, among three R. glutinosa chloroplast genome sequences including one from GenBank. The phylogenetic analysis showed that these R. glutinosa were closely clustered with related Rehmannia species, separated from other Orobanchaceae and Scrophulariaceae species.
Keywords: phylogeny, chloroplast genome, Rehmannia glutinosa
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