A novel and highly sensitive non-enzymatic glucose biosensor was developed by nucleating colloidal silver nanoparticles (Ag NPs) on MoS₂. The facile fabrication method, high reproducibility (97.5%) and stability indicates a promising capability for large-scale manufacturing. Additionally, the excellent sensitivity (9044.6 μA mM^-1 cm^-2), low detection limit (0.03 μM), appropriate linear range of 0.1-1000 μM, and high selectivity, suggests that this biosensor has a great potential to be applied for noninvasive glucose detection in human body fluids, such as sweat and saliva.