It is estimated that in developing countries, 60% or more of waste is lignocellulosic material that can be used in recycling processes. The purpose of this research has been aimed to find enzymatic cocktails from bacteria native to Ecuador that allow the degradation of lignocellulosic material, through the search of microorganisms collected in the Andean, Amazon and Antarctic regions, which can be introduced in a process of enzymatic hydrolysis of bagasse at  industrial scale. For this, qualitative and quantitative assays were carried out to measure the endoglucanase, exoglucanase and filter paper activity of the microorganisms and their enzymatic cocktails, as well as assays of enzymatic hydrolysis of the sugarcane. In addition, the value of the investments for Bacillus sp bacterium enzymatic cocktail production, and the total production cost were calculated.

It was identified that Peniccillium sp., Was the species with the highest activity of filter paper, showing 0.0073 UPF and a glucose yield of 11.968 in 100 grams of bagasse, which opens up the possibility of its use, in industrial processes. From this best strain of microorganism, the equipment was sized on an industrial scale and it was concluded that the investment cost would benefit the country.

The study shows that it is possible to generate a proprietary technology for the production of cellulolytic enzyme crudes in Ecuador.