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Robert YL Wang     Institute, Department or Faculty Head 
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Robert YL Wang published an article in May 2018.
Top co-authors See all
Chung-Ming Chang

10 shared publications

Research Center for Emerging Viral Infections, Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Kwei-Shan, Taoyuan 333, Taiwan

Scott C. Schuyler

10 shared publications

Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan

Chia Yin Chong

7 shared publications

Infectious Disease Service, Department of Pediatric Medicine, KK Women's and Children's Hospital, 100 Bukit Timah Road, 229899, Singapore

Ruey-Yi Chang

7 shared publications

Department of Life Science, National Dong Hwa University, Hualien, Taiwan, ROC

Justin Jang Hann Chu

5 shared publications

Laboratory of Molecular RNA Virology and Antiviral Strategies, Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System, 5 Science Drive 2, National University of Singapore, 117597, Singapore

Publication Record
Distribution of Articles published per year 

Total number of journals
published in
Article 8 Reads 0 Citations Circulating Salivary miRNA hsa-miR-221 as Clinically Validated Diagnostic Marker for Hand, Foot, and Mouth Disease in Pe... Nyo Min, Previtha Dawn Sakthi Vale, Anng Anng Wong, Natalie ... Published: 10 May 2018
EBioMedicine, doi: 10.1016/j.ebiom.2018.05.006
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
Enhancements in the diagnostic capabilities using host biomarkers are currently much needed where sensitivity and specificity issues plague the diagnosis of Hand, Foot and Mouth Disease (HFMD) in pediatrics clinical samples. We investigated miRNome profiles of HFMD saliva samples against healthy children and developed miRNA-based diagnosis models. Our 6-miRNA scoring model predicted HFMD with an overall accuracy of 85.11% in the training set and 92.86% in the blinded test set of Singapore cohort. Blinded evaluation of the model in Taiwan HFMD cases resulted in 77.08% accuracy with the 6-miRNA model and 68.75% with the 4-miRNA model. The strongest predictor of HFMD in all of the panels, hsa-miR-221 was found to be consistently and significantly downregulated in all of our HFMD cohorts. This is the first study to prove that HFMD infection could be diagnosed by circulating miRNAs in patient's saliva. Moreover, this study also serves as a stepping stone towards the future development of other infectious disease diagnosis workflows using novel biomarkers.
Article 3 Reads 3 Citations DnaJ homolog Hdj2 Facilitates Japanese Encephalitis Virus Replication Robert Yung-Liang Wang, Yu-Ru Huang, Ka-Man Chong, Chun-Yu H... Published: 14 October 2011
Virology Journal, doi: 10.1186/1743-422X-8-471
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
Japanese encephalitis virus (JEV) is a member of the mosquito-borne Flaviviridae family of viruses that causes human encephalitis. Upon infection of a new host, replication of viral RNA involves not only the viral RNA-dependent RNA polymerase (RdRp), but also host proteins. Host factors involved in JEV replication are not well characterized. We identified Hdj2, a heat-shock protein 40 (Hsp40)/DnaJ homolog, from a mouse brain cDNA library interacting with JEV nonstructural protein 5 (NS5) encoding viral RdRp using yeast two-hybrid system. Specific interaction of Hdj2 with NS5 was confirmed by coimmunoprecipitation and colocalization in JEV-infected cells. Overexpression of Hdj2 in JEV-infected cells led to an increase of RNA synthesis, and the virus titer was elevated approximately 4.5- to 10-fold. Knocking down of Hdj2 by siRNA reduced the virus production significantly. We conclude that Hdj2 directly associates with JEV NS5 and facilitates viral replication. This study is the first to demonstrate Hdj2 involved in JEV replication, providing insight into a potential therapeutic target and cell-based vaccine development of JEV infection.
Article 0 Reads 0 Citations Correction: Japanese encephalitis virus co-opts the ER-stress response protein GRP78 for viral infectivity Yi-Ping Wu, Chung-Ming Chang, Chun-Yu Hung, Meng-Chieh Tsai,... Published: 05 July 2011
Virology Journal, doi: 10.1186/1743-422X-8-338
DOI See at publisher website ABS Show/hide abstract
While reviewing the proof of our article [Virol J 2011, 8:128] after it was published on-line in its provisional form, we noticed two incidences where sentences in our article share a high level of similarity with two previously published papers.
Article 0 Reads 21 Citations Japanese encephalitis virus co-opts the ER-stress response protein GRP78 for viral infectivity Yi-Ping Wu, Chung-Ming Chang, Chun-Yu Hung, Meng-Chieh Tsai,... Published: 20 March 2011
Virology Journal, doi: 10.1186/1743-422X-8-128
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
The serum-free medium from Japanese encephalitis virus (JEV) infected Baby Hamster Kidney-21 (BHK-21) cell cultures was analyzed by liquid chromatography tandem mass spectrometry (LC-MS) to identify host proteins that were secreted upon viral infection. Five proteins were identified, including the molecular chaperones Hsp90, GRP78, and Hsp70. The functional role of GRP78 in the JEV life cycle was then investigated. Co-migration of GRP78 with JEV particles in sucrose density gradients was observed and co-localization of viral E protein with GRP78 was detected by immunofluorescence analysis in vivo. Knockdown of GRP78 expression by siRNA did not effect viral RNA replication, but did impair mature viral production. Mature viruses that do not co-fractionate with GPR78 displayed a significant decrease in viral infectivity. Our results support the hypothesis that JEV co-opts host cell GPR78 for use in viral maturation and in subsequent cellular infections.