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(2008 - 2017)
(2008 - 2017)
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Article 1 Read 0 Citations Endothelial cells regulate β -catenin activity in adrenocortical cells via secretion of basic fibroblast growth factor Published: 01 February 2017
Molecular and Cellular Endocrinology, doi: 10.1016/j.mce.2016.11.015
Endothelial cell-derived products influence the synthesis of aldosterone and cortisol in human adrenocortical cells by modulating proteins such as steroidogenic acute-regulatory (StAR) protein, steroidogenic factor (SF)-1 and CITED2. However, the potential endothelial cell-derived factors that mediate this effect are still unknown. The current study was perfomed to look into the control of β-catenin activity by endothelial cell-derived factors and to identify a mechanism by which they affect β-catenin activity in adrenocortical NCIH295R cells. Using reporter gene assays and Western blotting, we found that endothelial cell-conditioned medium (ECCM) led to nuclear translocation of β-catenin and an increase in β-catenin-dependent transcription that could be blocked by U0126, an inhibitor of the mitogen-activated protein kinase pathway. Furthermore, we found that a receptor tyrosin kinase (RTK) was involved in ECCM-induced β-catenin-dependent transcription. Through selective inhibition of RTK using Su5402, it was shown that receptors responding to basic fibroblast growth factor (bFGF) mediate the action of ECCM. Adrenocortical cells treated with bFGF showed a significant greater level of bFGF mRNA. In addition, HUVECs secrete bFGF in a density-dependent manner. In conclusion, the data suggest that endothelial cells regulate β-catenin activity in adrenocortical cells also via secretion of basic fibroblast growth factor.
Article 0 Reads 0 Citations Epitope-specific antitumor immunity suppresses tumor spread in papillary thyroid cancer Published: 18 November 2016
The Journal of Clinical Endocrinology & Metabolism, doi: 10.1210/jc.2016-2469
Context:Papillary thyroid cancer (PTC) is characterized by a lymphocytic infiltration. PTC patients with lymphocytic infiltration may have a better clinical outcome.Objective:Characterization of tumor epitope-specific immunity and correlation analyses with the clinical outcome.Patients:150 PTC patients; 40 Hashimoto’s thyroiditis (HT) patients; 21 healthy controls; 27.239 healthy Caucasians (for HLA typing).Main outcome measures:HLA class I restricted thyroperoxidase (TPO) and thyroglobulin (Tg) epitope-specific T cells (tetramer analyses), correlation analyses between HLA class II phenotypes, T cell immunity, and the clinical course.Results:The frequency of TPO- and Tg-specific CD8+ T cells in PTC patients was largely increased compared to healthy controls (TPO and Tg: p < 0.005 and p < 0.005) and was similar to those in HT patients. HLA-DQB1*03 positive PTC patients had a significantly lower risk (RR: 0.170, 95% CI: 0.037 to 0.755, p < 0.05) and HLA-DRB1*03 positive and HLA-DQB1*02 positive PTC patients a significantly higher risk (HLA-DRB1*03: RR: 4.400, 95% CI: 1.378 to 14.05, p < 0.05; HLA-DQB1*02: RR: 3.692, 95% CI: 1.102 to 12.38, p < 0.05) for distant metastases, compared to patients with other haplotypes. HLA-DQB1*03 positive PTC patients revealed an increased responsiveness of tumor epitopes in vitro. These tumor epitope-specific CD8+ T cells were also found in lymph node metastases of HLA-DQB1*03 positive PTC patients.Conclusion:We demonstrate a tumor epitope-specific immunity in PTC patients and the protective role of HLA-DQB1*03 against metastatic spread. These results have direct implications for new treatment options with immune check point inhibitors.
Article 0 Reads 0 Citations Prozesscharakterisierung der dynamischen Gefriertrocknung in einem Feststoffmischer Published: 21 June 2016
Chemie Ingenieur Technik, doi: 10.1002/cite.201500149
Zur Prozesscharakterisierung der dynamischen Gefriertrocknung in einem Feststoffmischer wurde ein effektiver Wärmeübergangskoeffizient bestimmt und separat der spezifische Energieeintrag durch die Bewegung des Rührwerkzeugs berechnet. Durch die erzwungene Bewegung der Schüttung findet ein mechanischer Energieeintrag von bis zu 9,7 kW m−3 in die Schüttung statt, der zur Deckung der Sublimationsenthalpie dient und die Trocknungszeit weiter reduziert. Die Untersuchungen wurden mit Milch und einer Laktoselösung als Modellsubstanz durchgeführt.An effective heat transfer coefficient of the dynamic freeze drying process in a solids mixer was determined as a crucial step in process characterization. The mechanical energy input due to the agitation action was calculated independently. Values of up to 9.7 kW m−3 were found for the mechanical energy input due the enforced motion. This energy input contributes to covering the required sublimation enthalpy and reduces the drying time. Trials were conducted using milk and a lactose solution as model substances.
BOOK-CHAPTER 0 Reads 0 Citations Speech Matters – Psychological Aspects of Artificial versus Anthropomorphic System Voices in User-Companion Interaction Published: 01 January 2016
Lecture Notes in Computer Science, doi: 10.1007/978-3-319-40406-6_30
Article 0 Reads 0 Citations Cost Optimal Retrofitting of Shopping Malls in Europe Published: 01 November 2015
Energy Procedia, doi: 10.1016/j.egypro.2015.11.223
The aim of this study was to assess the possible retrofitting actions with a cost-optimal vision for shopping mall managers. Cost optimization was analysed with state-of-the-art evaluation tools, taking initial, operational and maintenance into account. Costs of retrofitting measures were collected and with energy saving measures correlated.For each building the energy demand and primary energy (PE) savings for a wide number of packages representing “current practices”, “best practices” and “state of art solutions” were calculated. From the results for all reference buildings the best performing parameters with respect to primary energy savings and net present values were identified.
Article 0 Reads 1 Citation The Key Drivers for Energy Retrofitting of European Shopping Centres Published: 01 November 2015
Energy Procedia, doi: 10.1016/j.egypro.2015.11.368
This paper has as its main focus to define the drivers of retrofitting shopping centres. The drivers will provide the basis for developing energy retrofitting concepts, offering amongst other things, constructive technology, an understanding of typical functional patterns and socio-cultural aspects and an understanding of potentials associated with interaction with local energy grids. The primary focus of the study is energy use, but the fields of facilities, functions, management, ergonomics, safety and logistics are also studied, because sustainable shopping centres in addition to being energy efficient are commercially viable, accessible social arenas for all sections of society.