Please login first
Gou-Jen Wang   Professor  University Educator/Researcher 
Timeline See timeline
Gou-Jen Wang published an article in September 2018.
Research Keywords & Expertise See all
0 A
0 Aluminum
0 Gold
0 PDMS
0 PLA
0 Silicon
Top co-authors See all
Lewis R. Roberts

435 shared publications

From the Department of Radiology (D.E.J., S.M.T., K.A.B., B.E.K., J.L.A., M.R.C., D.A.W.) and Division of Gastroenterology and Hepatology (L.R.R.), Mayo Clinic School of Medicine, Mayo Clinic, 200 First St SW, Rochester, MN 55905; and Department of Health Sciences Research, Mayo Clinic School of Medicine, Jacksonville, Fla (R.E.C.).

Rudolf Holze

338 shared publications

AG Elektrochemie, Institut für Chemie, Technische Universität Chemnitz, D-09107 Chemnitz, Germany

Yong-Gui Chen

238 shared publications

Key Laboratory of Geotechnical and Underground Engineering of Ministry of Education, Department of Geotechnical Engineering, Tongji University, Shanghai, People’s Republic of China

Jiangang Sun

71 shared publications

Argonne National Laboratory, Argonne, Illinois 60439, USA

Bin Wang

65 shared publications

Institute of Chemical Materials, China Academy of Engineering Physics, Chengdu, Sichuan 621900, China

48
Publications
33
Reads
0
Downloads
126
Citations
Publication Record
Distribution of Articles published per year 
(1999 - 2018)
Publications See all
Article 0 Reads 0 Citations Chemical-Free Extraction of Functional Mitochondria Using a Microfluidic Device Yu-Han Hsiao, Ching-Wen Li, Jui-Chih Chang, Sung-Tzu Chen, C... Published: 27 September 2018
Inventions, doi: 10.3390/inventions3040068
DOI See at publisher website
ABS Show/hide abstract
This paper proposes the use of a chip-based microfluidic device to extract functional and chemical free mitochondria. A simple microfluidic device was designed and fabricated. An osteosarcoma cybrid cell line was employed to demonstrate the efficiency of the proposed microfluidic device. The membrane proteins (mitochondrial complex I-V and Tom20) and morphology of the extracted mitochondria were examined by Western blot and transmission electron microscopy (TEM), respectively. The purity and mitochondrial membrane potential of the extracted mitochondria were individually measured by 10-N-alkyl acridine orange and tetramethylrhodamine ethyl ester staining via flow cytometry. Experimental results revealed that expressed pattern of complex I–V in device-extracted mitochondria was close to that of mitochondria in total cell lysis and device extraction significantly prevented chemical modification of complex IV protein via a conventional kit, although device extract similar amounts of mitochondria to the conventional kit revealed by Tom20 expression. Furthermore, purity of device-extracted mitochondria was above 93.7% and mitochondria still retained normal activity after device extraction proven by expression of mitochondrial membrane potential as well as the entire mitochondrial morphology. These results confirmed that the proposed microfluidic device could obtain functional mitochondria without structural damage.
Article 0 Reads 0 Citations Design and manufacture of high-filling-efficiency microfluidic devices Chun-Te Wu, Gou-Jen Wang Published: 01 May 2018
The International Journal of Advanced Manufacturing Technology, doi: 10.1007/s00170-018-2039-1
DOI See at publisher website
Article 5 Reads 0 Citations Using Anodic Aluminum Oxide Film and Nanoimprint to Produce Polymer Anti-counterfeit Labels Chang-Yi Peng, Che-Wei Hsu, Ching-Wen Li, Gou-Jen Wang Published: 06 June 2017
Smart Science, doi: 10.1080/23080477.2017.1336153
DOI See at publisher website
Article 2 Reads 0 Citations An endothelial cultured condition medium embedded porous PLGA scaffold for the enhancement of mouse embryonic stem cell ... Ching-Wen Li, Wei-Ting Pan, Jyh-Cherng Ju, Gou-Jen Wang Published: 12 April 2016
Biomedical Materials, doi: 10.1088/1748-6041/11/2/025015
DOI See at publisher website
PubMed View at PubMed
Article 3 Reads 0 Citations Pelvic-perineal reconstruction with the combined transverse upper gracilis and profunda artery perforator (TUG-PAP) flap Pedro Ciudad, Rory Dower, Fabio Nicoli, Georgios Orfaniotis,... Published: 01 April 2016
Journal of Plastic, Reconstructive & Aesthetic Surgery, doi: 10.1016/j.bjps.2015.11.019
DOI See at publisher website
PubMed View at PubMed
Article 4 Reads 0 Citations The Micro/Nanohybrid Structures Enhancing B35 Cell Guidance on Chitosan Ying-Ting Lin, Ching-Wen Li, Gou-Jen Wang Published: 17 March 2016
Journal of Nanotechnology in Engineering and Medicine, doi: 10.1115/1.4032602
DOI See at publisher website
Top