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Gou-Jen Wang   Professor  University Educator/Researcher 
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Gou-Jen Wang published an article in September 2018.
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Top co-authors See all
Shie-Liang Hsieh

311 shared publications

Genomics Research Center, Academia Sinica, Taipei, Taiwan

Shan-Hui Hsu

205 shared publications

Institute of Polymer Science and Engineering; National Taiwan University; Taipei 10617 Taiwan Republic of China

Rafael V. Davalos

151 shared publications

Department of Biomedical Engineering and Mechanics, Virginia Tech, 329 ICTAS Stanger St (0298), Blacksburg, VA 24061, USA

Shekhar Bhansali

60 shared publications

Engineering, Florida International University

Wenjea J. Tseng

50 shared publications

Department of Materials Science and Engineering, National Chung Hsing University, Taichung 402, Taiwan

Publication Record
Distribution of Articles published per year 
(2001 - 2017)
Total number of journals
published in
Publications See all
Article 0 Reads 0 Citations Chemical-Free Extraction of Functional Mitochondria Using a Microfluidic Device Yu-Han Hsiao, Ching-Wen Li, Jui-Chih Chang, Sung-Tzu Chen, C... Published: 27 September 2018
Inventions, doi: 10.3390/inventions3040068
DOI See at publisher website ABS Show/hide abstract
This paper proposes the use of a chip-based microfluidic device to extract functional and chemical free mitochondria. A simple microfluidic device was designed and fabricated. An osteosarcoma cybrid cell line was employed to demonstrate the efficiency of the proposed microfluidic device. The membrane proteins (mitochondrial complex I-V and Tom20) and morphology of the extracted mitochondria were examined by Western blot and transmission electron microscopy (TEM), respectively. The purity and mitochondrial membrane potential of the extracted mitochondria were individually measured by 10-N-alkyl acridine orange and tetramethylrhodamine ethyl ester staining via flow cytometry. Experimental results revealed that expressed pattern of complex I–V in device-extracted mitochondria was close to that of mitochondria in total cell lysis and device extraction significantly prevented chemical modification of complex IV protein via a conventional kit, although device extract similar amounts of mitochondria to the conventional kit revealed by Tom20 expression. Furthermore, purity of device-extracted mitochondria was above 93.7% and mitochondria still retained normal activity after device extraction proven by expression of mitochondrial membrane potential as well as the entire mitochondrial morphology. These results confirmed that the proposed microfluidic device could obtain functional mitochondria without structural damage.
PROCEEDINGS-ARTICLE 0 Reads 0 Citations Sciatic Nerve Regeneration in Mice Using A PLGA Microgroove Patterned Conduit Fills with Microfiber Ching-Wen Li, Hui-Yu Hsu, Yu-Fen Chung, Jong-Hang Chen, Gou-... Published: 01 August 2018
Proceedings of the 4th World Congress on Electrical Engineering and Computer Systems and Science, doi: 10.11159/icbes18.131
DOI See at publisher website
Article 0 Reads 0 Citations Design and manufacture of high-filling-efficiency microfluidic devices Chun-Te Wu, Gou-Jen Wang Published: 01 May 2018
The International Journal of Advanced Manufacturing Technology, doi: 10.1007/s00170-018-2039-1
DOI See at publisher website
Article 0 Reads 0 Citations Template-assisted fabrication of tunable aspect ratio, biocompatible iron oxide pillar arrays Ryan Chang Tseng, Ching-Wen Li, Gou-Jen Wang Published: 02 April 2018
Advanced Materials Letters, doi: 10.5185/amlett.2018.1842
DOI See at publisher website
Article 0 Reads 1 Citation Flexible Photonic Crystal Material for Multiple Anticounterfeiting Applications Chang-Yi Peng, Che-Wei Hsu, Ching-Wen Li, Po-Lin Wang, Chien... Published: 21 March 2018
ACS Applied Materials & Interfaces, doi: 10.1021/acsami.8b00292
DOI See at publisher website
Article 0 Reads 2 Citations Control of cell proliferation by a porous chitosan scaffold with multiple releasing capabilities Shu-Jyun Cai, Ching-Wen Li, Daphne Weihs, Gou-Jen Wang Published: 31 December 2017
Science and Technology of Advanced Materials, doi: 10.1080/14686996.2017.1406287
DOI See at publisher website ABS Show/hide abstract
The aim of this study was to develop a porous chitosan scaffold with long-acting drug release as an artificial dressing to promote skin wound healing. The dressing was fabricated by pre-freezing at different temperatures (−20 and −80 °C) for different periods of time, followed by freeze-drying to form porous chitosan scaffolds with different pore sizes. The chitosan scaffolds were then used to investigate the effect of the controlled release of fibroblast growth factor-basic (bFGF) and transforming growth factor-β1 (TGFβ1) on mouse fibroblast cells (L929) and bovine carotid endothelial cells (BEC). The biocompatibility of the prepared chitosan scaffold was confirmed with WST-1 proliferation and viability assay, which demonstrated that the material is suitable for cell growth. The results of this study show that the pore sizes of the porous scaffolds prepared by freeze-drying can change depending on the pre-freezing temperature and time via the formation of ice crystals. In this study, the scaffolds with the largest pore size were found to be 153 ± 32 μm and scaffolds with the smallest pores to be 34 ± 9 μm. Through cell culture analysis, it was found that the concentration that increased proliferation of L929 cells for bFGF was 0.005 to 0.1 ng/mL, and the concentration for TGFβ1 was 0.005 to 1 ng/mL. The cell culture of the chitosan scaffold and growth factors shows that 3.75 ng of bFGF in scaffolds with pore sizes of 153 ± 32 μm can promote L929 cell proliferation, while 400 pg of TGFβ1 in scaffolds with pore size of 34 ± 9 μm can enhance the proliferation of L929 cells, but also inhibit BEC proliferation. It is proposed that the prepared chitosan scaffolds can form a multi-drug (bFGF and TGFβ1) release dressing that has the ability to control wound healing via regulating the proliferation of different cell types.