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Christophe HANO     Senior Scientist or Principal Investigator 
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Christophe HANO published an article in March 2019.
Top co-authors See all
Alain Favre-Réguillon

99 shared publications

Univ Lyon Laboratoire de Génie des Procédés Catalytiques (UMR 5285) CPE Lyon; 43 boulevard du 11 Novembre 1918 F-69100 Villeurbanne France

Bilal Haider Abbasi

91 shared publications

Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan

David Giron

50 shared publications

Université de Tours

Eric Gontier

45 shared publications

Unité de Recherche EA3900 BIOPI-UPJV Biologie des plantes et Innovation, Université de Picardie Jules Verne, UFR des Sciences, Ilot des poulies, 33 rue Saint Leu, 80039 Amiens Cedex 1 - France

Randolph Rj Arroo

38 shared publications

Faculty of Health and Life Sciences, De Montfort University, The Gateway, Leicester LE1 9BH, UK;(A.S.B.);(R.R.J.A.)

Publication Record
Distribution of Articles published per year 
(2005 - 2019)
Total number of journals
published in
PREPRINT 0 Reads 0 Citations Interactive Effects of Light and Melatonin on Biosynthesis of Silymarin and Anti-inflammatory Potential in Callus Cultur... Muzamil Shah, Muhammad Asad Ullah, Samantha Drouet, Muhammad... Published: 01 March 2019
doi: 10.20944/preprints201903.0007.v1
DOI See at publisher website ABS Show/hide abstract
Silybum marianum L. is a well-known medicinal herb, primarily used in liver protection. Light strongly affects several physiological processes along with secondary metabolites biosynthesis in plants. Herein, S. marianum was exploited for in vitro potential under different light regimes in the presence of melatonin. The optimum callogenic response occurred in combination of 1.0 mg/L α-naphthalene acetic acid and 0.5 mg/L 6-Benzylaminopurine under photoperiod. Continuous light associated with melatonin treatment increased total flavonoid content (TFC), total phenolic content (TPC) and antioxidant potential, followed by photoperiod and dark treatments. The increased level of melatonin has a synergistic effect on biomass accumulation under continuous light and photoperiod, while adverse effect was observed under dark condition. More detailed phytochemical analysis showed maximum total silymarin content (11.92 mg/g DW) when placed under continuous light + 1.0 mg/L melatonin. Individually, the level of silybins (A and B), silydianin, isolsilychristin and silychristin was found highest under continuous light. Anti-inflammatory activities were also studied and highest percent inhibition was recorded against 15-LOX for cultures cultivated under continuous light (42.33%). The current study helps to better understand the influence of melatonin and different light regimes on silymarin production as well as antioxidant and anti-inflammatory activities in S. marianum callus extracts.
Article 2 Reads 8 Citations Microwave-Assisted Extraction of Herbacetin Diglucoside from Flax (Linum usitatissimum L.) Seed Cakes and Its Quantifica... Ophélie Fliniaux, Cyrielle Corbin, Aina Ramsay, Sullivan Ren... Published: 10 March 2014
Molecules, doi: 10.3390/molecules19033025
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
Flax (Linum usitatissimum L.) seeds are widely used for oil extraction and the cold-pressed flaxseed (or linseed) cakes obtained during this process constitute a valuable by-product. The flavonol herbacetin diglucoside (HDG) has been previously reported as a constituent of the flaxseed lignan macromolecule linked through ester bonds to the linker molecule hydroxymethylglutaric acid. In this context, the development and validation of a new approach using microwave-assisted extraction (MAE) of HDG from flaxseed cakes followed by quantification with a reverse-phase HPLC system with UV detection was purposed. The experimental parameters affecting the HDG extraction yield, such as microwave power, extraction time and sodium hydroxide concentration, from the lignan macromolecule were optimized. A maximum HDG concentration of 5.76 mg/g DW in flaxseed cakes was measured following an irradiation time of 6 min, for a microwave power of 150 W using a direct extraction in 0.1 M NaOH in 70% (v/v) aqueous methanol. The optimized method was proven to be rapid and reliable in terms of precision, repeatability, stability and accuracy for the extraction of HDG. Comparison with a conventional extraction method demonstrated that MAE is more effective and less time-consuming.
Article 0 Reads 1 Citation Isolation of nuclear proteins from flax (Linum usitatissimum L.) seed coats for gene expression regulation studies Sullivan Renouard, Corbin Cyrielle, Tatiana Lopez, Frédéric ... Published: 09 January 2012
BMC Research Notes, doi: 10.1186/1756-0500-5-15
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
While seed biology is well characterized and numerous studies have focused on this subject over the past years, the regulation of seed coat development and metabolism is for the most part still non-elucidated. It is well known that the seed coat has an essential role in seed development and its features are associated with important agronomical traits. It also constitutes a rich source of valuable compounds such as pharmaceuticals. Most of the cell genetic material is contained in the nucleus; therefore nuclear proteins constitute a major actor for gene expression regulation. Isolation of nuclear proteins responsible for specific seed coat expression is an important prerequisite for understanding seed coat metabolism and development. The extraction of nuclear proteins may be problematic due to the presence of specific components that can interfere with the extraction process. The seed coat is a rich source of mucilage and phenolics, which are good examples of these hindering compounds. In the present study, we propose an optimized nuclear protein extraction protocol able to provide nuclear proteins from flax seed coat without contaminants and sufficient yield and quality for their use in transcriptional gene expression regulation by gel shift experiments. Routinely, around 250 μg of nuclear proteins per gram of fresh weight were extracted from immature flax seed coats. The isolation protocol described hereafter may serve as an effective tool for gene expression regulation and seed coat-focused proteomics studies.
Article 0 Reads 40 Citations Differential accumulation of monolignol-derived compounds in elicited flax (Linum usitatissimum) cell suspension culture... Christophe Hano, M. Addi, L. Bensaddek, D. Cronier, S. Balto... Published: 16 November 2005
Planta, doi: 10.1007/s00425-005-0156-1
DOI See at publisher website PubMed View at PubMed