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Petra S. Dittrich      
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Petra S. Dittrich published an article in December 2018.
Top co-authors
Dieter Seebach

307 shared publications

Laboratorium für Organische Chemie, Departement Chemie und Angewandte Biowissenschaften, ETH-Zürich, Hönggerberg HCI, Vladimir-Prelog-Weg 3, CH-8093 Zürich, Switzerland

Peter Walde

210 shared publications

Department of Materials, ETH Zurich, Vladimir-Prelog-Weg 5, 8093 Zurich, Switzerland

Beata Kolesinska

71 shared publications

Institute of Organic Chemistry, Lodz University of Technology, Żeromskiego 116, 90-924 Lodz, Poland

Martin Pabst

12 shared publications

Laboratory of Organic Chemistry, Department of Chemistry and Applied Biosciences, ETH Zurich, CH-8093 Zurich, Switzerland

Yanlong Xing

1 shared publications

Leibniz-Institut für Analytische Wissenschaften—ISAS—e. V, 12489 Berlin, Germany

Publication Record
Distribution of Articles published per year 
(2010 - 2018)
Total number of journals
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Publications See all
Article 1 Read 0 Citations Microfluidic Platform for Multimodal Analysis of Enzyme Secretion in Nanoliter Droplet Arrays Dominik Haidas, Simon Bachler, Martin Köhler, Lars M. Blank,... Published: 20 December 2018
Analytical Chemistry, doi: 10.1021/acs.analchem.8b04506
DOI See at publisher website
Article 0 Reads 0 Citations Tunable Membrane Potential Reconstituted in Giant Vesicles Promotes Permeation of Cationic Peptides at Nanomolar Concent... Chao-Chen Lin, Michael Bachmann, Simon Bachler, Koushik Venk... Published: 19 November 2018
ACS Applied Materials & Interfaces, doi: 10.1021/acsami.8b12217
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We investigate the influence of membrane potential on the permeation of cationic peptides. Therefore, we employ a microfluidic chip capable of capturing giant unilamellar vesicles (GUVs) in physical traps and fast exchange of chemical compounds. Control experiments with calcein proved that the vesicle membranes’ integrity is not affected by the physical traps and applied shear forces. Combined with fluorescence correlation spectroscopy, permeation of fluorescently labeled peptides across vesicle membranes can be measured down to the nanomolar level. With the addition of a lipophilic ruthenium(II) complex Ru(C17)22+, GUVs consisting of mixed acyl phospholipids are prepared with a negative membrane potential, resembling the membrane asymmetry in cells. The membrane potential serves as a driving force for the permeation of cationic cell-penetrating peptides (CPPs) nonaarginine (Arg9) and the human immunodeficiency virus trans-activator of transcription (TAT) peptide already at nanomolar doses. Hyperpolarization of the membrane by photo-oxidation of Ru(C17)22+ enhances permeation significantly from 55 to 78% for Arg9. This specific enhancement for Arg9 (cf. TAT) is ascribed to the higher affinity of the arginines to the phosphoserine head groups. On the other hand, permeation is decreased by introducing an additional negative charge in close proximity to the N-terminal arginine residue when changing the fluorophore. In short, with the capability to reconstitute membrane potential as well as shear stress, our system is a suitable platform for modeling the membrane permeability of pharmaceutics candidates. The results also highlight the membrane potential as a major cause of discrepancies between vesicular and cellular studies on CPP permeation.
Article 3 Reads 1 Citation Influence of the Membrane Dye R18 and of DMSO on Cell Penetration of Guanidinium-Rich Peptides Felix Kurth, Petra. S. Dittrich, Peter Walde, Dieter Seebach Published: 21 September 2018
Chemistry & Biodiversity, doi: 10.1002/cbdv.201800302
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A quantitative analysis by confocal fluorescence microscopy of the entry into HEK293 and MCF-7 cells by fluorescein-labeled octaarginine (1) and by three octa-Adp derivatives (2 – 4, octamers of the β-Asp-Arg-dipeptide, derived from the biopolymer cyanophycin) is described, including the effects of the membrane dye R18 and of DMSO on cell penetration.
Article 0 Reads 1 Citation Pneumolysin-damaged cells benefit from non-homogeneous toxin binding to cholesterol-rich membrane domains Patrick Drücker, Simon Bachler, Heidi Wolfmeier, Roman Schoe... Published: 01 August 2018
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, doi: 10.1016/j.bbalip.2018.04.010
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Nucleated cells eliminate lesions induced by bacterial pore-forming toxins, such as pneumolysin via shedding patches of damaged plasmalemma into the extracellular milieu. Recently, we have shown that the majority of shed pneumolysin is present in the form of inactive pre-pores. This finding is surprising considering that shedding is triggered by Ca2+-influx following membrane perforation and therefore is expected to positively discriminate for active pores versus inactive pre-pores.
Article 0 Reads 2 Citations High-Throughput Monitoring of Cocaine and Its Metabolites in Hair Using Microarrays for Mass Spectrometry and Matrix-Ass... Angéline Kernalléguen, Robert Steinhoff, Simon Bachler, Petr... Published: 22 January 2018
Analytical Chemistry, doi: 10.1021/acs.analchem.7b04693
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Article 1 Read 3 Citations One-Dimensional Nanostructures: Microfluidic-Based Synthesis, Alignment and Integration towards Functional Sensing Devic... Yanlong Xing, Petra S. Dittrich Published: 05 January 2018
Sensors, doi: 10.3390/s18010134
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Microfluidic-based synthesis of one-dimensional (1D) nanostructures offers tremendous advantages over bulk approaches e.g., the laminar flow, reduced sample consumption and control of self-assembly of nanostructures. In addition to the synthesis, the integration of 1D nanomaterials into microfluidic chips can enable the development of diverse functional microdevices. 1D nanomaterials have been used in applications such as catalysts, electronic instrumentation and sensors for physical parameters or chemical compounds and biomolecules and hence, can be considered as building blocks. Here, we outline and critically discuss promising strategies for microfluidic-assisted synthesis, alignment and various chemical and biochemical applications of 1D nanostructures. In particular, the use of 1D nanostructures for sensing chemical/biological compounds are reviewed.