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Javier Del Campo   Dr.  Senior Scientist or Principal Investigator 
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Javier Del Campo published an article in May 2013.
Top co-authors See all
J-Y. Hihn

41 shared publications

Université de Franche-Comté, Besançon, France

Stelian Lupu

21 shared publications

Department of Analytical Chemistry and Environmental Engineering, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street 1-5, 011061, Bucharest, Romania

Paul Catalin Balaure

19 shared publications

Department of Organic Chemistry, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street 1-5, 011061, Bucharest, Romania

Constantin Mihailciuc

13 shared publications

Department of Physical Chemistry, Faculty of Chemistry, University of Bucharest, Bld. Regina Elisabeta 4-12, 030018, Bucharest, Romania

Boris Lakard

10 shared publications

Institut UTINAM, UMR 6214 CNRS/Université de Franche-Comté, Besançon, France

Publication Record
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Article 0 Reads 10 Citations Development of amperometric biosensors based on nanostructured tyrosinase-conducting polymer composite electrodes. Stelian Lupu, Cecilia Lete, Paul Cătălin Balaure, Dan Ion Ca... Published: 21 May 2013
Sensors, doi: 10.3390/s130506759
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
Bio-composite coatings consisting of poly(3,4-ethylenedioxythiophene) (PEDOT) and tyrosinase (Ty) were successfully electrodeposited on conventional size gold (Au) disk electrodes and microelectrode arrays using sinusoidal voltages. Electrochemical polymerization of the corresponding monomer was carried out in the presence of various Ty amounts in aqueous buffered solutions. The bio-composite coatings prepared using sinusoidal voltages and potentiostatic electrodeposition methods were compared in terms of morphology, electrochemical properties, and biocatalytic activity towards various analytes. The amperometric biosensors were tested in dopamine (DA) and catechol (CT) electroanalysis in aqueous buffered solutions. The analytical performance of the developed biosensors was investigated in terms of linear response range, detection limit, sensitivity, and repeatability. A semi-quantitative multi-analyte procedure for simultaneous determination of DA and CT was developed. The amperometric biosensor prepared using sinusoidal voltages showed much better analytical performance. The Au disk biosensor obtained by 50 mV alternating voltage amplitude displayed a linear response for DA concentrations ranging from 10 to 300 μM, with a detection limit of 4.18 μM.