Please login first
Francois Mesnard   Professor  University Educator/Researcher 
Timeline See timeline
Francois Mesnard published an article in February 2019.
Top co-authors See all
Christophe Hano

161 shared publications

Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan

Catherine Deborde

71 shared publications

UMR1332 Biologie du Fruit et Pathologie, INRA, Centre INRA de Bordeaux, Villenave d’Ornon, France

Eric Gontier

45 shared publications

Unité de Recherche EA3900 BIOPI-UPJV Biologie des plantes et Innovation, Université de Picardie Jules Verne, UFR des Sciences, Ilot des poulies, 33 rue Saint Leu, 80039 Amiens Cedex 1 - France

Randolph R. J. Arroo

38 shared publications

Faculty of Health and Life Sciences, De Montfort University, The Gateway, Leicester LE1 9BH, UK;(A.S.B.);(R.R.J.A.)

Clotilde Ferroud

29 shared publications

Equipe de Chimie Moléculaire, EA 7341, Laboratoire de Chimie Moléculaire, Génie des Procédés Chimiques et Energétiques, Conservatoire National des Arts et Métiers, 2 rue Conté, Paris 75003, France

1 Followers
26
Publications
3
Reads
0
Downloads
71
Citations
Publication Record
Distribution of Articles published per year 
(1997 - 2019)
Total number of journals
published in
 
22
 
Publications See all
Article 0 Reads 0 Citations Optimizing 1D 1H-NMR profiling of plant samples for high throughput analysis: extract preparation, standardization, auto... Catherine Deborde, Jean-Xavier Fontaine, Daniel Jacob, Adolf... Published: 26 February 2019
Metabolomics, doi: 10.1007/s11306-019-1488-3
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
Proton nuclear magnetic resonance spectroscopy (1H-NMR)-based metabolomic profiling has a range of applications in plant sciences. The aim of the present work is to provide advice for minimizing uncontrolled variability in plant sample preparation before and during NMR metabolomic profiling, taking into account sample composition, including its specificity in terms of pH and paramagnetic ion concentrations, and NMR spectrometer performances. An automation of spectrometer preparation routine standardization before NMR acquisition campaign was implemented and tested on three plant sample sets (extracts of durum wheat spikelet, Arabidopsis leaf and root, and flax leaf, root and stem). We performed 1H-NMR spectroscopy in three different sites on the wheat sample set utilizing instruments from two manufacturers with different probes and magnetic field strengths. The three collections of spectra were processed separately with the NMRProcFlow web tool using intelligent bucketing, and the resulting buckets were subjected to multivariate analysis. Comparability of large- (Arabidopsis) and medium-size (flax) datasets measured at 600 MHz and from the wheat sample set recorded at the three sites (400, 500 and 600 MHz) was exceptionally good in terms of spectral quality. The coefficient of variation of the full width at half maximum (FWHM) and the signal-to-noise ratio (S/N) of two selected peaks was comprised between 5 and 10% depending on the size of sample set and the spectrometer field. EDTA addition improved citrate and malate resonance patterns for wheat sample sets. A collection of 22 samples of wheat spikelet extracts was used as a proof of concept and showed that the data collected at the three sites on instruments of different field strengths and manufacturers yielded the same discrimination pattern of the biological groups. Standardization or automation of several steps from extract preparation to data reduction improves data quality for small to large collections of plant samples of different origins. The online version of this article (10.1007/s11306-019-1488-3) contains supplementary material, which is available to authorized users.
Article 0 Reads 0 Citations MuSeeQ, a novel supervised image analysis tool for the simultaneous phenotyping of the soluble mucilage and seed morphom... Fabien Miart, Jean-Xavier Fontaine, Christophe Pineau, Hervé... Published: 18 December 2018
Plant Methods, doi: 10.1186/s13007-018-0377-5
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
The mucilage is a model to study the polysaccharide biosynthesis since it is produced in large amounts and composed of complex polymers. In addition, it is of great economic interest for its technical and nutritional value. A fast method for phenotyping the released mucilage and the seed morphometric parameters will be useful for fundamental, food, pharmaceutical and breeding researches. Current strategies to phenotype soluble mucilage are restricted to visual evaluations or are highly time-consuming. Here, we developed a high-throughput phenotyping method for the simultaneous measurement of the soluble mucilage content released on a gel and the seed morphometric parameters. Within this context, we combined a biochemical assay and an open-source computer-aided image analysis tool, MuSeeQ. The biochemical assay consists in sowing seeds on an agarose medium containing the dye toluidine blue O, which specifically stains the mucilage once it is released on the gel. The second part of MuSeeQ is a macro developed in ImageJ allowing to quickly extract and analyse 11 morphometric data of seeds and their respective released mucilages. As an example, MuSeeQ was applied on a flax recombinant inbred lines population (previously screened for fatty acids content.) and revealed significant correlations between the soluble mucilage shape and the concentration of some fatty acids, e.g. C16:0 and C18:2. Other fatty acids were also found to correlate with the seed shape parameters, e.g. C18:0 and C18:2. MuSeeQ was then showed to be used for the analysis of other myxospermous species, including Arabidopsis thaliana and Camelina sativa. MuSeeQ is a low-cost and user-friendly method which may be used by breeders and researchers for phenotyping simultaneously seeds of specific cultivars, natural variants or mutants and their respective soluble mucilage area released on a gel. The script of MuSeeQ and video tutorials are freely available at http://MuSeeQ.free.fr. The online version of this article (10.1186/s13007-018-0377-5) contains supplementary material, which is available to authorized users.
Article 0 Reads 2 Citations Insight into the Influence of Cultivar Type, Cultivation Year, and Site on the Lignans and Related Phenolic Profiles, an... Laurine Garros, Samantha Drouet, Cyrielle Corbin, Cédric Dec... Published: 14 October 2018
Molecules, doi: 10.3390/molecules23102636
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
Flaxseeds are a functional food representing, by far, the richest natural grain source of lignans, and accumulate substantial amounts of other health beneficial phenolic compounds (i.e., flavonols, hydroxycinnamic acids). This specific accumulation pattern is related to their numerous beneficial effects on human health. However, to date, little data is available concerning the relative impact of genetic and geographic parameters on the phytochemical yield and composition. Here, the major influence of the cultivar over geographic parameters on the flaxseed phytochemical accumulation yield and composition is evidenced. The importance of genetic parameters on the lignan accumulation was further confirmed by gene expression analysis monitored by RT-qPCR. The corresponding antioxidant activity of these flaxseed extracts was evaluated, both in vitro, using ferric reducing antioxidant power (FRAP), oxygen radical absorbance capacity (ORAC), and iron chelating assays, as well as in vivo, by monitoring the impact of UV-induced oxidative stress on the lipid membrane peroxidation of yeast cells. Our results, both the in vitro and in vivo studies, confirm that flaxseed extracts are an effective protector against oxidative stress. The results point out that secoisolariciresinol diglucoside, caffeic acid glucoside, and p-coumaric acid glucoside are the main contributors to the antioxidant capacity. Considering the health benefits of these compounds, the present study demonstrates that the flaxseed cultivar type could greatly influence the phytochemical intakes and, therefore, the associated biological activities. We recommend that this crucial parameter be considered in epidemiological studies dealing with flaxseeds.
Article 0 Reads 3 Citations A multi-omics analysis of the regulatory changes induced by miR-223 in a monocyte/macrophage cell line Eléonore M'baya-Moutoula, Loïc Louvet, Roland Molinie, Ida C... Published: 01 August 2018
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, doi: 10.1016/j.bbadis.2018.05.010
DOI See at publisher website
Article 0 Reads 0 Citations In Vivo Use of 1D and 2D 1H NMR to Examine the Glycosylation of Scopoletin in Duboisia myoporoides Cell Suspensions Ophélie Fliniaux, Albrecht Roscher, Dominique Cailleu, Franç... Published: 14 June 2018
Planta Medica, doi: 10.1055/a-0632-2249
DOI See at publisher website
Article 0 Reads 1 Citation Investigation of Linum flavum (L.) Hairy Root Cultures for the Production of Anticancer Aryltetralin Lignans Sullivan Renouard, Cyrielle Corbin, Samantha Drouet, Barbara... Published: 26 March 2018
International Journal of Molecular Sciences, doi: 10.3390/ijms19040990
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
Linum flavum hairy root lines were established from hypocotyl pieces using Agrobacterium rhizogenes strains LBA 9402 and ATCC 15834. Both strains were effective for transformation but induction of hairy root phenotype was more stable with strain ATCC 15834. Whereas similar accumulation patterns were observed in podophyllotoxin-related compounds (6-methoxy-podophyllotoxin, podophyllotoxin and deoxypodophyllotoxin), significant quantitative variations were noted between root lines. The influence of culture medium and various treatments (hormone, elicitation and precursor feeding) were evaluated. The highest accumulation was obtained in Gamborg B5 medium. Treatment with methyl jasmonate, and feeding using ferulic acid increased the accumulation of aryltetralin lignans. These results point to the use of hairy root culture lines of Linum flavum as potential sources for these valuable metabolites as an alternative, or as a complement to Podophyllum collected from wild stands.
Top