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Antonio Galvez   Professor  Institute, Department or Faculty Head 
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Antonio Galvez published an article in April 2019.
Top co-authors See all
Oscar P. Kuipers

282 shared publications

Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands

Hikmate Abriouel

173 shared publications

1Área de Microbiología, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentales, Universidad de Jaén, Jaén, Spain

Ákos T. Kovács

81 shared publications

Bacterial Interactions and Evolution Group, DTU Bioengineering, Technical University of Denmark, Søltofts Plads Building 221, Kongens Lyngby 2800, Denmark

Isabel Prieto

70 shared publications

2Área de Fisiología, Departamento de Ciencias de la Salud, Universidad de Jaén, Paraje de Las Lagunillas s/n, Jaén 23072, Spain;(I.P.);(A.B.S.);(M.R.)

Rubén Pérez-Pulido

61 shared publications

Microbiology Division, Department of Health Sciences, University of Jaen, Jaen, Spain

231
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Distribution of Articles published per year 
(1991 - 2019)
Total number of journals
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32
 
Publications See all
Article 0 Reads 0 Citations Biocide tolerance and antibiotic resistance of Enterobacter spp. isolated from Algerian hospital environment Zakaria Boutarfi, Sid-Ahmed Rebiahi, Touhami Morghad, Ruben ... Published: 18 April 2019
Journal of Global Antimicrobial Resistance, doi: 10.1016/j.jgar.2019.04.005
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In the present study, 77Enterobacter strains from a collection of 175 Gram-negative bacilli isolated from Tlemcen University Hospital Center (North-West of Algeria) were tested for antimicrobial resistance, biocide tolerance and genetic determinants of antimicrobial resistance. Strains were identified by 16S rDNA sequencing. Biocide tolerance was determined by broth microdilution. Antibiotic resistance was determined by disk diffusion. Genetic determinants of resistance were studied by PCR amplification with suitable primers. The most predominantEnterobacter species was E. cloacae (58.4%) followed by E. hormaechei (24.7%). The most common antimicrobal resistance was for ticarcillin singly or in combination with clavulanic acid (70%) followed by cefepime (68.8%) cefotaxime (63.6%), gentamicin (54.5%) and ceftazidime (50.6%). Tobramycin was active on 87% of the strains. Levels of biocide tolerance were high for hexachorophene, and to a less extent also for benzalkonium chloride. Extended-spectrum β-lactamase genes blaTEM, blaCTX-M were detected in 44.2% and 36.4% of the strains, respectively. Other antibiotic resistance genes frequently detected were aac(6´)Ib (57.1%) and sul2 (45.5%). Multiply-resistant strains carrying several antimicrobial resistance genes were frequent. Significant positive correlations were detected for efflux pumps with antimicrobial resistance genes and between antimicrobial resistance genes as well. Results of the study reveal that theEnterobacter strains from hospital settings are both resistant to clinically-used antimicrobials and tolerant to biocides. Biocide tolerance could be an advantage for prevalence of antibiotic-resistant strains in hospitals.
Article 0 Reads 0 Citations Copper tolerance and antibiotic resistance in soil bacteria from olive tree agricultural fields routinely treated with c... Nicolás Glibota, Mª José Grande Burgos, Antonio Gálvez, Elen... Published: 24 March 2019
Journal of the Science of Food and Agriculture, doi: 10.1002/jsfa.9708
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Article 0 Reads 1 Citation Refined versus Extra Virgin Olive Oil High-Fat Diet Impact on Intestinal Microbiota of Mice and Its Relation to Differen... Nieves Martínez, Isabel Prieto, Marina Hidalgo, Ana Belén Se... Published: 23 February 2019
Microorganisms, doi: 10.3390/microorganisms7020061
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Extra virgin olive oil (EVOO) has been reported to have a distinct influence on gut microbiota in comparison to other fats, with its physiological benefits widely studied. However, a large proportion of the population consumes olive oil after a depurative process that not only mellows its taste, but also deprives it of polyphenols and other minority components. In this study, we compare the influence on the intestinal microbiota of a diet high in this refined olive oil (ROO) with other fat-enriched diets. Swiss Webster mice were fed standard or a high-fat diet enriched with EVOO, ROO, or butter (BT). Physiological parameters were also evaluated. At the end of the feeding period, DNA was extracted from feces and the 16S rRNA was pyrosequenced. The group fed ROO behaved differently to the EVOO group in half the families with statistically significant differences among the diets, with higher comparative levels in three families—Desulfovibrionaceae, Spiroplasmataceae, and Helicobacteraceae—correlating with total cholesterol. These results are again indicative of a link between specific diets, certain physiological parameters and the prevalence of some taxa, but also support the possibility that polyphenols and minor components of EVOO are involved in some of the proposed effects of this fat through the modulation of the intestinal microbiota
Article 0 Reads 0 Citations Effect of high hydrostatic pressure and activated film packaging on bacterial diversity of fruit puree María José Grande Burgos, Irene Ortega Blázquez, Rubén Pérez... Published: 01 February 2019
LWT, doi: 10.1016/j.lwt.2018.10.083
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Article 0 Reads 0 Citations Bacterial Inactivation by Using Plastic Materials Activated with Combinations of Natural Antimicrobials Irene Ortega Blázquez, María José Grande Burgos, Rubén Pérez... Published: 12 December 2018
Coatings, doi: 10.3390/coatings8120460
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Natural antimicrobials have gained interest as possible inhibitors of biofilm formation. The aim of the present study was to determine the efficacy of antimicrobials derived from essential oils (carvacrol, thymol) plus bacteriocin AS-48 immobilized on two plastic supports (low density polyethylene and polyethylene–polyamide films) on bacterial inactivation. The polyethylene–polyamide vacuum-packaging plastic film activated with a combination of thymol plus enterocin AS-48 was the most effective in reducing the concentrations of viable planktonic and sessile cells for Listeria innocua, Lactobacillus fructivorans, Bacillus coagulans, and Bacillus licheniformis. Results from the study highlight the potential of polyethylene–polyamide film activated with thymol plus enterocin AS-48 for reducing the viable cell concentrations of spoilage Gram-positive bacteria and Listeria in both planktonic and sessile states.
Article 0 Reads 0 Citations Deciphering Resistome and Virulome Diversity in a Porcine Slaughterhouse and Pork Products Through Its Production Chain Guillermo Campos Calero, Natacha Caballero Gómez, Nabil Beno... Published: 12 September 2018
Frontiers in Microbiology, doi: 10.3389/fmicb.2018.02099
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We aimed to better understand resistome and virulome patterns on animal and process-area surfaces through a pig slaughterhouse to track possible contamination within the food production chain. Culture-dependent methods revealed high levels of microbial contamination, corresponding to mesophilic and pathogenic bacteria on both the animal and process-area surfaces mainly in the anesthesia (AA and AS) zone followed by “scorching and whip” (FA and FS) zone and also in the end products. To evaluate the potential risk of antibiotic resistance and virulence determinants, shotgun metagenomic DNA-sequencing of isolates from selected areas/products uncovered a high diversity and richness of antibiotic resistance genes (ARGs): 55–62 genes in the anesthesia area (AA and AS) and 35–40 in “animal-arrival zone” (MA and MS). The “scorching and whip” (FA and FS) area, however, exhibited lowered abundance of ARGs (1–6), indicating that the scalding and depilating process (an intermediate zone between “anesthesia” and “scorching and whip”) significantly decreased bacterial load by 1–3 log10 but also diminished the resistome. The high prevalence of antibiotic-inactivating enzyme genes in the “animal-arrival zone” (60–65%) and “anesthesia” area (56%) were mainly represented by those for aminoglycoside (46–51%) and lincosamide (14–19%) resistance, which did not reflect selective pressures by antibiotics most commonly used in pig therapy—tetracyclines and beta-lactams. Contrary to ARGs, greater number of virulence resistance genes were detected after evisceration in some products such as kidney, which reflected the poor hygienic practices. More than 19 general virulence features—mainly adherence, secretion system, chemotaxis and motility, invasion and motility were detected in some products. However, immune evasion determinants were detected in almost all samples analyzed from the beginning of the process, with highest amounts found from the anesthesia area. We conclude that there are two main sources of contamination in a pig slaughterhouse: the microorganisms carried on the animals’ hide, and those from the evisceration step. As such, focussing control measures, e.g., enhanced disinfection procedures, on these contamination-source areas may reduce risks to food safety and consumer health, since the antibiotic and virulence determinants may spread to end products and the environment; further, ARG and virulence traits can exacerbate pathogen treatments.
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