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W. Rudolf Seitz   Dr.   
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W. Rudolf Seitz published an article in April 2018.
Top co-authors
Leila F. Deravi

12 shared publications

Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts 02115, United States

Alex Papantones

1 shared publications

Boston Analytical, 14 Manor Parkway, Salem, NH 03079, USA

W. Rudolf Seitz

1 shared publications

Department of Chemistry, University of New Hampshire, Durham NH 03824, USA;(A.T.);(R.Y.);(J.C.)

Publication Record
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Article 1 Read 0 Citations Rapid, High Affinity Binding by a Fluorescein Templated Copolymer Combining Covalent, Hydrophobic, and Acid–Base Noncova... Casey J. Grenier, Anthony Timberman, Rongfang Yang, John Cso... Published: 25 April 2018
Sensors, doi: 10.3390/s18051330
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
A new type of biomimetic templated copolymer has been prepared by reverse addition fragmentation chain transfer polymerization (RAFT) in dioxane. The initial formulation includes the template fluorescein, N-isopropylacrylamide (NIPAM, 84 mol %), methacrylic acid (MAA, 5-mol %), 4-vinylpyridine (4-VP, 9 mmol %), and N,N′-methylenebis(acrylamide) (MBA, 2 mol %). PolyNIPAM is a thermosensitive polymer that comes out of aqueous solution above its lower critical solution temperature forming hydrophobic ‘crosslinks’. MAA and 4-VP interact in dioxane forming acid–base crosslinks. The excess 4-VP serves as a recognition monomer organizing around the template fluorescein to form a binding site that is held in place by the noncovalent and covalent crosslinks. The MBA is a covalent crosslinker. The RAFT agent in the resulting copolylmer was reduced to a thiol and attached to gold nanoparticles. The gold nanoparticle bound copolymer binds fluorescein completely in less than two seconds with an affinity constant greater than 108 M−1. A reference copolymer prepared with the same monomers by the same procedure binds fluorescein much more weakly.