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(2008 - 2018)
(2008 - 2018)
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Article 0 Reads 0 Citations Selection, Characterization, and Application of ssDNA Aptamer against Furaneol Published: 30 November 2018
Molecules, doi: 10.3390/molecules23123159
Furaneol is an aroma compound which occurs naturally in foods and is used as an artificial flavor. Detection of furaneol is required in food science and food processing industry. Capture- Systematic Evolution of Ligands by EXponential enrichment (SELEX) protocol was applied for the isolation of an aptamer binding to furaneol, a small volatile organic substance contributing to the flavor of various products. Thirteen cycles of selection were performed. The resulting DNA pool was cloned, using blunt-end cloning, and ninety-six plasmids were sequenced and analyzed. Eight oligonucleotides were selected as aptamer candidates and screened for the ability to bind to furaneol, using three different methods—magnetic-beads associated elution assay, SYBR Green I assay, and exonuclease protection assay. One of the candidates was further characterized as an aptamer. The apparent equilibrium constant was determined to be (1.1 ± 0.4) µM, by the fluorescent method. The reported aptamer was applied for development of the ion-sensitive field-effect transistor (ISFET)-based biosensor, for the analysis of furaneol, in the concentration range of 0.1–10 µM.
Article 9 Reads 1 Citation Amplified Detection of the Aptamer–Vanillin Complex with the Use of Bsm DNA Polymerase Published: 26 December 2017
Sensors, doi: 10.3390/s18010049
The electrochemical detection of interactions between aptamers and low-molecular-weight targets often lacks sensitivity. Signal amplification improves the detection of the aptamer-analyte complex; Bsm DNA polymerase was used to amplify the signal from the interaction of vanillin and its aptamer named Van_74 on an ion-sensitive field-effect transistor (ISFET)-based biosensor. The aptamer was immobilized on the ISFET sensitive surface. A short DNA probe was hybridized with the aptamer and dissociated from it upon vanillin addition. A free probe interacted with a special DNA molecular beacon initiated the Bsm DNA polymerase reaction that was detected by ISFET. A buffer solution suitable for both aptamer action and Bsm DNA polymerase activity was determined. The ISFET was shown to detect the Bsm DNA polymerase reaction under the selected conditions. Vanillin at different concentrations (1 × 10-6-1 × 10-8 M) was detected using the biosensor with signal amplification. The developed detection system allowed for the determination of vanillin, starting at a 10-8 M concentration. Application of the Bsm DNA polymerase resulted in a 15.5 times lower LoD when compared to the biosensor without signal amplification (10.1007/s00604-017-2586-4).
Article 0 Reads 3 Citations Aptamer based vanillin sensor using an ion-sensitive field-effect transistor Published: 02 December 2017
Microchimica Acta, doi: 10.1007/s00604-017-2586-4
Article 0 Reads 1 Citation Field-effect transition sensor for KI detection based on self-assembled calixtube monolayers Published: 01 December 2017
Biosensors and Bioelectronics, doi: 10.1016/j.bios.2017.06.050
Article 4 Reads 0 Citations Amplified Detection of the Aptamer—Vanillin Complex with the Use of Bsm DNA Polymerase Published: 30 November 2017
Proceedings, doi: 10.3390/proceedings1080848
The decreased sensitivity for electrochemical detection of low-molecular-weight targets with the use of aptamers is a great problem of scientific importance.
Article 5 Reads 3 Citations Fabrication and package of ISFET biosensor for micro volume analysis with the use of direct ink writing approach Published: 01 March 2017
Materials Science in Semiconductor Processing, doi: 10.1016/j.mssp.2016.12.007