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Antonio J. Ricco  - - - 
Top co-authors
Richard C. Quinn

10 shared publications

Carl Sagan Center, SETI Institute, NASA Ames Research Center, Moffett Field, California 94035, United States

Nigel J. Kent

7 shared publications

Advanced Processing Technology Research Centre, Dublin City University, Dublin, Ireland

Jose Luis Garcia-Cordero

6 shared publications

CINVESTAV-Monterrey

Andreas Elsaesser

5 shared publications

Leiden Observatory, Leiden University, P.O. Box 9513, NL-2300 RA Leiden, The Netherlands; s:(A.E.);(P.E.)

John W. Hines

3 shared publications

NASA Ames Research Center, Moffett Field, California

146
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804
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Publication Record
Distribution of Articles published per year 
(1985 - 2016)
Total number of journals
published in
 
29
 
Publications See all
Article 0 Reads 1 Citation Blood group alters platelet binding kinetics to von Willebrand factor and consequently platelet function Eimear Dunne, Qin M. Qi, Eric S. Shaqfeh, Jamie M. O'sulliva... Published: 14 January 2019
Blood, doi: 10.1182/blood-2018-06-855528
DOI See at publisher website
Article 0 Reads 1 Citation Dynamic platelet function is markedly different in patients with cancer compared to healthy donors Jonathan Cowman, Louis Richter, Roisin Walsh, Niamh Keegan, ... Published: 25 September 2018
Platelets, doi: 10.1080/09537104.2018.1513475
DOI See at publisher website
Article 0 Reads 0 Citations Obtaining Chemical Selectivity from a Single, Nonselective Sensing Film: Two-Stage Adaptive Estimation Scheme with Multi... Karthick Sothivelr, Florian Bender, Fabien Josse, Edwin E. Y... Published: 17 August 2018
ACS Sensors, doi: 10.1021/acssensors.8b00353
DOI See at publisher website
Article 0 Reads 1 Citation Payload hardware and experimental protocol development to enable future testing of the effect of space microgravity on t... A.C. Matin, J.-H. Wang, Mimi Keyhan, Rachna Singh, Michael B... Published: 01 November 2017
Life Sciences in Space Research, doi: 10.1016/j.lssr.2017.05.001
DOI See at publisher website
Article 0 Reads 4 Citations Microgravity validation of a novel system for RNA isolation and multiplex quantitative real time PCR analysis of gene ex... Macarena Parra, Jimmy Jung, Travis D. Boone, Luan Tran, Eliz... Published: 06 September 2017
PLOS ONE, doi: 10.1371/journal.pone.0183480
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
The International Space Station (ISS) National Laboratory is dedicated to studying the effects of space on life and physical systems, and to developing new science and technologies for space exploration. A key aspect of achieving these goals is to operate the ISS National Lab more like an Earth-based laboratory, conducting complex end-to-end experimentation, not limited to simple microgravity exposure. Towards that end NASA developed a novel suite of molecular biology laboratory tools, reagents, and methods, named WetLab-2, uniquely designed to operate in microgravity, and to process biological samples for real-time gene expression analysis on-orbit. This includes a novel fluidic RNA Sample Preparation Module and fluid transfer devices, all-in-one lyophilized PCR assays, centrifuge, and a real-time PCR thermal cycler. Here we describe the results from the WetLab-2 validation experiments conducted in microgravity during ISS increment 47/SPX-8. Specifically, quantitative PCR was performed on a concentration series of DNA calibration standards, and Reverse Transcriptase-quantitative PCR was conducted on RNA extracted and purified on-orbit from frozen Escherichia coli and mouse liver tissue. Cycle threshold (Ct) values and PCR efficiencies obtained on-orbit from DNA standards were similar to Earth (1 g) controls. Also, on-orbit multiplex analysis of gene expression from bacterial cells and mammalian tissue RNA samples was successfully conducted in about 3 h, with data transmitted within 2 h of experiment completion. Thermal cycling in microgravity resulted in the trapping of gas bubbles inside septa cap assay tubes, causing small but measurable increases in Ct curve noise and variability. Bubble formation was successfully suppressed in a rapid follow-up on-orbit experiment using standard caps to pressurize PCR tubes and reduce gas release during heating cycles. The WetLab-2 facility now provides a novel operational on-orbit research capability for molecular biology and demonstrates the feasibility of more complex wet bench experiments in the ISS National Lab environment.
Article 2 Reads 3 Citations Platelet behaviour on von Willebrand Factor changes in pregnancy: Consequences of haemodilution and intrinsic changes in... Jonathan Cowman, Sieglinde Müllers, Eimear Dunne, Adam Ralph... Published: 25 July 2017
Scientific Reports, doi: 10.1038/s41598-017-06959-6
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
Platelet function in pregnancy is poorly understood. Previous studies of platelet function in pregnancy have used non-physiological assays of platelet function with conflicting results. This study using a physiological assay of platelet function investigated platelet interactions with von Willebrand Factor (VWF) in blood from healthy pregnant women and healthy non-pregnant controls. Blood samples (200 µl) from third-trimester pregnancies (n = 21) and non-pregnant controls (n = 21) were perfused through custom-made parallel-plate flow chambers coated with VWF under arterial shear (1,500 s−1). Multi-parameter measurements of platelet interactions with the immobilized VWF surface were recorded by digital-image microscopy and analysed using custom-designed platelet-tracking software. Platelet interactions with VWF decreased in healthy third-trimester pregnant participants relative to controls. This effect is most likely due to haemodilution which occurs physiologically during pregnancy. Interestingly, platelets in blood from pregnant participants translocated more slowly on VWF under arterial-shear conditions. These decreases in platelet translocation speed were independent of haemodilution, suggesting intrinsic changes in platelet function with pregnancy.
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