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Valentina Carabelli  - - - 
Top co-authors See all
Emilio Carbone

139 shared publications

Department of Drug Science, NIS CentreUniversity of Torino Torino Italy

Emanuele Enrico

56 shared publications

Istituto Nazionale di Ricerca Metrologica, Torino, Piemonte, ITALY

Jacopo Forneris

41 shared publications

Istituto Nazionale di Fisica Nucleare Sezione di Torino, TO, Italy, Torino, ITALY

Paolo Olivero

38 shared publications

Universita degli Studi di Torino Dipartimento di Fisica, Torino, Piemonte, ITALY

Federico Picollo

34 shared publications

Section of Torino, Istituto Nazionale di Fisica Nucleare (INFN), Torino, Italy

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Distribution of Articles published per year 
(1994 - 2019)
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29
 
Publications See all
Article 0 Reads 0 Citations Quantal Release of Dopamine and Action Potential Firing Detected in Midbrain Neurons by Multifunctional Diamond-Based Mi... Giulia Tomagra, Federico Picollo, Alfio Battiato, Barbara Pi... Published: 09 April 2019
Frontiers in Neuroscience, doi: 10.3389/fnins.2019.00288
DOI See at publisher website ABS Show/hide abstract
Micro-Graphitic Single Crystal Diamond Multi Electrode Arrays (μG-SCD-MEAs) have so far been used as amperometric sensors to detect catecholamines from chromaffin cells and adrenal gland slices. Besides having time resolution and sensitivity that are comparable with carbon fiber electrodes, that represent the gold standard for amperometry, μG-SCD-MEAs also have the advantages of simultaneous multisite detection, high biocompatibility and implementation of amperometric/potentiometric protocols, aimed at monitoring exocytotic events and neuronal excitability. In order to adapt diamond technology to record neuronal activity, the μG-SCD-MEAs in this work have been interfaced with cultured midbrain neurons to detect electrical activity as well as quantal release of dopamine (DA). μG-SCD-MEAs are based on graphitic sensing electrodes that are embedded into the diamond matrix and are fabricated using MeV ion beam lithography. Two geometries have been adopted, with 4 × 4 and 8 × 8 microelectrodes (20 μm × 3.5 μm exposed area, 200 μm spacing). In the amperometric configuration, the 4 × 4 μG-SCD-MEAs resolved quantal exocytosis from midbrain dopaminergic neurons. KCl-stimulated DA release occurred as amperometric spikes of 15 pA amplitude and 0.5 ms half-width, at a mean frequency of 0.4 Hz. When used as potentiometric multiarrays, the 8 × 8 μG-SCD-MEAs detected the spontaneous firing activity of midbrain neurons. Extracellularly recorded action potentials (APs) had mean amplitude of ∼-50 μV and occurred at a mean firing frequency of 0.7 Hz in 67% of neurons, while the remaining fired at 6.8 Hz. Comparable findings were observed using conventional MEAs (0.9 and 6.4 Hz, respectively). To test the reliability of potentiometric recordings with μG-SCD-MEAs, the D2-autoreceptor modulation of firing was investigated by applying levodopa (L-DOPA, 20 μM), and comparing μG-SCD-MEAs, conventional MEAs and current-clamp recordings. In all cases, L-DOPA reduced the spontaneous spiking activity in most neurons by 70%, while the D2-antagonist sulpiride reversed this effect. Cell firing inhibition was generally associated with increased APs amplitude. A minority of neurons was either insensitive to, or potentiated by L-DOPA, suggesting that AP recordings originate from different midbrain neuronal subpopulations and reveal different modulatory pathways. Our data demonstrate, for the first time, that μG-SCD-MEAs are multi-functional biosensors suitable to resolve real-time DA release and AP firing in in vitro neuronal networks.
BOOK-CHAPTER 1 Read 0 Citations Diamond-Based Multi Electrode Arrays for Monitoring Neurotransmitter Release Giulia Tomagra, Alfio Battiato, Ettore Bernardi, Alberto Pas... Published: 18 January 2019
Strukturelle Phasenanalyse von chemischen Prozessadditiven in der Silikat-Industrie, doi: 10.1007/978-3-030-04324-7_17
DOI See at publisher website ABS Show/hide abstract
In the present work, we report on the fabrication of a diamond-based device targeted to the detection of quantal neurotransmitter release. We have developed Multi-electrode Arrays with 16 independent graphitic channels fabricated by means of Deep Ion Beam Lithography (DIBL). These devices are capable of detecting the in vitro exocytotic event from neurosecretory cells, while overcoming several critical limitations of standard amperometric techniques.
Article 0 Reads 0 Citations p140Cap Regulates GABAergic Synaptogenesis and Development of Hippocampal Inhibitory Circuits. Isabella Russo, Daniela Gavello, Elisabetta Menna, David Van... Published: 01 January 2019
Cerebral Cortex, doi: 10.1093/cercor/bhx306
DOI See at publisher website PubMed View at PubMed
PROCEEDINGS-ARTICLE 0 Reads 0 Citations Color centres in diamond from single photon sources to ODMR in cells Marco Genovese, Ekaterina Moreva, Paolo Traina, Jacopo Forne... Published: 11 September 2018
Quantum Photonic Devices 2018, doi: 10.1117/12.2323102
DOI See at publisher website
BOOK-CHAPTER 4 Reads 0 Citations Boron-Doped Diamond and Graphitic Multiarrays for Neurotransmitter Sensing Alberto Pasquarelli, Federico Picollo, Valentina Carabelli Published: 13 May 2018
Optical Nano- and Microsystems for Bioanalytics, doi: 10.1007/5346_2018_24
DOI See at publisher website
Article 2 Reads 3 Citations Nanodiamonds-induced effects on neuronal firing of mouse hippocampal microcircuits L. Guarina, C. Calorio, D. Gavello, E. Moreva, P. Traina, A.... Published: 02 February 2018
Scientific Reports, doi: 10.1038/s41598-018-20528-5
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
Fluorescent nanodiamonds (FND) are carbon-based nanomaterials that can efficiently incorporate optically active photoluminescent centers such as the nitrogen-vacancy complex, thus making them promising candidates as optical biolabels and drug-delivery agents. FNDs exhibit bright fluorescence without photobleaching combined with high uptake rate and low cytotoxicity. Focusing on FNDs interference with neuronal function, here we examined their effect on cultured hippocampal neurons, monitoring the whole network development as well as the electrophysiological properties of single neurons. We observed that FNDs drastically decreased the frequency of inhibitory (from 1.81 Hz to 0.86 Hz) and excitatory (from 1.61 to 0.68 Hz) miniature postsynaptic currents, and consistently reduced action potential (AP) firing frequency (by 36%), as measured by microelectrode arrays. On the contrary, bursts synchronization was preserved, as well as the amplitude of spontaneous inhibitory and excitatory events. Current-clamp recordings revealed that the ratio of neurons responding with AP trains of high-frequency (fast-spiking) versus neurons responding with trains of low-frequency (slow-spiking) was unaltered, suggesting that FNDs exerted a comparable action on neuronal subpopulations. At the single cell level, rapid onset of the somatic AP (“kink”) was drastically reduced in FND-treated neurons, suggesting a reduced contribution of axonal and dendritic components while preserving neuronal excitability.
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