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Esin Akçael  - - - 
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Selma Ozturk

7 shared publications

TÜBİTAK, The Scientific and Technological Research Council of Turkey, Marmara Research Center, Genetic Engineering and Biotechnology Institute, Gebze, Kocaeli 41400, Turkey

Özlem Ertekin

2 shared publications

Harun Kocaağa

1 shared publications

3
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Distribution of Articles published per year 
(2013 - 2018)
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published in
 
3
 
Publications
Article 0 Reads 0 Citations Biological Activity of the Carrier as a Factor in Immunogen Design for Haptens Özlem Ertekin, Esin Akçael, Harun Kocaağa, Selma Öztürk Published: 14 November 2018
Molecules, doi: 10.3390/molecules23112977
DOI See at publisher website ABS Show/hide abstract
Immunoanalytical methods are frequently employed in the detection of hazardous small molecular weight compounds. However, antibody development for these molecules is a challenge, because they are haptens and cannot induce a humoral immune response in experimental animals. Immunogenic forms of haptens are usually prepared by conjugating them to a protein carrier which serves as an immune stimulator. However, the carrier is usually considered merely as a bulk mass, and its biological activity is ignored. Here, we induced an endocytic receptor, transferrin receptor, by selecting its ligand as a carrier protein to enhance antibody production. We conjugated aflatoxin, a potent carcinogenic food contaminant, to transferrin and evaluated its potential to stimulate antibody production with respect to ovalbumin conjugates. Transferrin conjugates induced aflatoxin-specific immune responses in the second immunization, while ovalbumin conjugates reached similar antibody titers after 5 injections. Monoclonal antibodies were successfully developed with mice immunized with either of the conjugates.
Article 0 Reads 0 Citations Monoclonal antibody development for quantitative analysis of pancreatitis-associated protein Ilham Bahhar, Ozlem Ertekin, Seyda Pirincci, Bengu Ergenoglu... Published: 01 August 2017
Journal of Biotechnology, doi: 10.1016/j.jbiotec.2017.06.971
DOI See at publisher website
Article 0 Reads 1 Citation Antibody based systems for the determination of mycotoxins in food and feed Özlem Ertekin, Fatma Betul Guloglu, Seyda Pirincci, Sevecen ... Published: 01 July 2013
Current Opinion in Biotechnology, doi: 10.1016/j.copbio.2013.05.028
DOI See at publisher website
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