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Oscar P. Kuipers  - - - 
Top co-authors See all
Willem M De Vos

474 shared publications

Laboratory of Microbiology, Wageningen University, 6708WE Wageningen, The Netherlands

José-Antonio Bengoechea

251 shared publications

Wellcome-Wolfson Institute for Experimental Medicine, Queen's University Belfast, Belfast, United Kingdom

Antonio M. Gálvez

245 shared publications

Área de Microbiología, Departamento de Ciencias de la Salud, Universidad de Jaén, Paraje de Las Lagunillas s/n, Jaén 23072, Spain

Bert Poolman

230 shared publications

Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands

Ida J. Van Der Klei

206 shared publications

Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen

Publication Record
Distribution of Articles published per year 
(2002 - 2019)
Total number of journals
published in
Publications See all
Article 0 Reads 0 Citations Heterologous biosynthesis and characterization of a glycocin from a thermophilic bacterium Arnoldas Kaunietis, Andrius Buivydas, Donaldas J. Čitavičius... Published: 07 March 2019
Nature Communications, doi: 10.1038/s41467-019-09065-5
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
The genome of the thermophilic bacterium, Aeribacillus pallidus 8, encodes the bacteriocin pallidocin. It belongs to the small class of glycocins and is posttranslationally modified, containing an S-linked glucose on a specific Cys residue. In this study, the pallidocin biosynthetic machinery is cloned and expressed in Escherichia coli to achieve its full biosynthesis and modification. It targets other thermophilic bacteria with potent activity, demonstrated by a low minimum inhibitory concentration (MIC) value. Moreover, the characterized biosynthetic machinery is employed to produce two other glycopeptides Hyp1 and Hyp2. Pallidocin and Hyp1 exhibit antibacterial activity against closely related thermophilic bacteria and some Bacillus sp. strains. Thus, heterologous expression of a glycocin biosynthetic gene cluster including an S-glycosyltransferase provides a good tool for production of hypothetical glycocins encoded by various bacterial genomes and allows rapid in vivo screening.
Article 0 Reads 0 Citations Phosphosugar stress in Bacillus subtilis: Intracellular accumulation of mannose 6-phosphate derepresed the glcR-phoC ope... Kambiz Morabbi Heravi, Irfan Manzoor, Hildegard Watzlawick, ... Published: 19 February 2019
Journal of Bacteriology, doi: 10.1128/jb.00732-18
DOI See at publisher website
Article 0 Reads 0 Citations Metabolic engineering and synthetic biology employing Lactococcus lactis and Bacillus subtilis cell factories. Amanda Y Van Tilburg, Haojie Cao, Sjoerd B Van Der Meulen, A... Published: 18 February 2019
Current Opinion in Biotechnology, doi: 10.1016/j.copbio.2019.01.007
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Article 0 Reads 0 Citations Draft Genome Sequences of Six Bacillus Strains and One Brevibacillus Strain Isolated from the Rhizosphere of Perennial R... Zhibo Li, Chunxu Song, Anne De Jong, Oscar P. Kuipers Published: 24 January 2019
Microbiology Resource Announcements, doi: 10.1128/mra.01586-18
DOI See at publisher website PubMed View at PubMed ABS Show/hide abstract
Seven strains of endospore-forming bacteria with antagonistic activity against a series of plant pathogens were sequenced in order to investigate their antimicrobial gene clusters and antimicrobial modes of action. The selected strains include six Bacillus strains and one Brevibacillus strain.
Article 0 Reads 0 Citations Adaption to glucose limitation is modulated by the pleotropic regulator CcpA, independent of selection pressure strength Claire E. Price, Filipe Branco Dos Santos, Anne Hesseling, J... Published: 10 January 2019
BMC Evolutionary Biology, doi: 10.1186/s12862-018-1331-x
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A central theme in (micro)biology is understanding the molecular basis of fitness i.e. which strategies are successful under which conditions; how do organisms implement such strategies at the molecular level; and which constraints shape the trade-offs between alternative strategies. Highly standardized microbial laboratory evolution experiments are ideally suited to approach these questions. For example, prolonged chemostats provide a constant environment in which the growth rate can be set, and the adaptive process of the organism to such environment can be subsequently characterized. We performed parallel laboratory evolution of Lactococcus lactis in chemostats varying the quantitative value of the selective pressure by imposing two different growth rates. A mutation in one specific amino acid residue of the global transcriptional regulator of carbon metabolism, CcpA, was selected in all of the evolution experiments performed. We subsequently showed that this mutation confers predictable fitness improvements at other glucose-limited growth rates as well. In silico protein structural analysis of wild type and evolved CcpA, as well as biochemical and phenotypic assays, provided the underpinning molecular mechanisms that resulted in the specific reprogramming favored in constant environments. This study provides a comprehensive understanding of a case of microbial evolution and hints at the wide dynamic range that a single fitness-enhancing mutation may display. It demonstrates how the modulation of a pleiotropic regulator can be used by cells to improve one trait while simultaneously work around other limiting constraints, by fine-tuning the expression of a wide range of cellular processes. The online version of this article (10.1186/s12862-018-1331-x) contains supplementary material, which is available to authorized users.
Article 0 Reads 0 Citations Fluorescently Labeled DNA Interacts with Competence and Recombination Proteins and Is Integrated and Expressed Following... Mirjam Boonstra, Nina Vesel, Oscar P. Kuipers Published: 25 September 2018
mBio, doi: 10.1128/mbio.01161-18
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We used DNA that was covalently labeled with fluorescent nucleotides to investigate the transformation process of Bacillus subtilis at the molecular level. We show that the labeled DNA colocalizes with components of the competence machinery, the chromosome, and the recombination protein RecA. Using time-lapse microscopy and microfluidics, we visualized, in real-time, the uptake of fluorescently labeled DNA. We found that under these conditions, cell division is not required for the expression of integrated DNA. Because the competence machinery is conserved in naturally competent bacteria, this method can also be used to investigate the transformation process in many other bacterial species.