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José Antônio Rocha Gontijo  - - - 
Top co-authors See all
Augusto H Custódio

10 shared publications

State University of Campinas

Patrícia Aline Boer

5 shared publications

Universidade Estadual de Campinas

Heloisa Balan Assalin

3 shared publications

Universidade Estadual de Campinas

Leticia B. Sene

2 shared publications

Bioscience Institute at São Paulo State University (UNESP)

Barbara V Cardoso

1 shared publications

State University of Campinas

Publication Record
Distribution of Articles published per year 
(1998 - 2019)
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Publications See all
Article 0 Reads 0 Citations Effect of intracerebroventricular epinephrine microinjection on blood pressure and urinary sodium handling in gestationa... Bárbara Vaccari Cardoso, Augusto Henrique Custódio, Patrícia... Published: 15 April 2019
Biology Open, doi: 10.1242/bio.038562
DOI See at publisher website ABS Show/hide abstract
In this study, we hypothesized that blunting of the natriuresis response to intracerebroventricularly (ICV) microinjected adrenergic agonists is involved in the development of hypertension in maternal low-protein intake (LP) offspring. A stainless steel cannula was stereotaxically implanted into the right lateral ventricle (LV), then we evaluated the ICV administration of adrenergic agonists at increasing concentrations, and of α1 and α2-adrenoceptor antagonists on blood pressure and urinary sodium handling in LP offspring relative to an age-matched normal-protein intake (NP) group. We confirmed that epinephrine (Epi) microinjected into the LV of conscious NP rats leads to enhanced natriuresis followed by a reduction in arterial pressure. This response is associated with increased proximal and post-proximal sodium excretion accompanied by an unchanged glomerular filtration rate. The current study showed, in both NP and LP offspring, that the natriuretic effect of Epi injection into the LV was abolished by prior local microinjection of an α1-adrenoceptor antagonist (prazosin). Conversely, LV α2-adrenoceptor antagonist (yohimbine) administration potentiated the action of Epi. The LV yohimbine pretreatment normalized urinary sodium excretion and reduced the blood pressure in LP compared with age-matched NP offspring. These are, as far as we are aware, the first results showing the role of central adrenergic receptors’ interaction on hypertension pathogenesis in maternal LP fetal-programming offspring. This study also provides good evidence for the existence of central nervous system adrenergic mechanisms consisting of α1 and α2-adrenoceptors, which work reciprocally on the control of renal sodium excretion and blood pressure. Although the precise mechanism of the different natriuretic response of NP and LP rats is still uncertain, these results lead us to speculate that inappropriate neural adrenergic pathways might have significant effects on tubule sodium transport, resulting in the inability of the kidneys to control hydrosaline balance and, consequently, an increase in blood pressure.
PREPRINT-CONTENT 0 Reads 0 Citations miRNAs, target genes expression and morphological analysis on the heart in gestational protein-restricted offspring José A.R. Gontijo, Heloisa Balan Assalin, Patrícia Aline Boe... Published: 27 December 2018
bioRxiv, doi: 10.1101/507038
DOI See at publisher website ABS Show/hide abstract
BACKGROUND Gestational protein restriction was associated with low birth weight, hypertension and higher prevalence of cardiac disorders in adults. Several mechanisms, including epigenetics, could be related with the cardiovascular phenotype on protein-restricted offspring. Thus, we investigated the morphological cardiac effects of gestational protein restriction and left ventricle miRNAs and target genes expression pattern in both 12-day and 16-week old gestational protein-restricted male offspring. METHODS Pregnant Wistar rats were allocated into two groups, according to protein supply during pregnancy: NP (normal protein diet- 17%) or LP (low protein diet - 6%). The study evaluates the effects of maternal protein restriction on food consumption and body weight of both pregnant dams and offspring, systolic blood pressure in 16-wk old offspring and on cardiac morphometric and molecular parameters in both 12-d and 16-wk old offspring. RESULTS: Dams on the gestational protein-restricted diet had lower body weight gain and higher food intake. Gestational protein-restricted offspring had low birth weight, followed by rapidly body weight recovery, hypertension, and increased myocytes cross-sectional area and collagen fraction at 16-week old age. At 12-days old, miR-184, miR-192, miR-376c, miR-380-3p, miR-380-5p, miR-451, and miR-582-3p had increased expression, and miR-547 and miR-743a had decreased expression in the gestational protein-restricted left ventricle. At 16-week old, let-7b, miR-125a-3p, miR-142-3p, miR-182 and miR-188-5p had increased expression and let-7g, miR-107, miR-127, miR-181a, miR-181c, miR-184, miR-324-5p, miR-383, miR-423-5p and miR-484 had decreased expression in gestational protein-restricted left ventricle. Target predicted gene expression analysis shown higher expression of Dnmt3a, Oxct1, Rictor and Trps1 and lower expression of Bbs1 and Calml3 in 12-day old protein-restricted offspring. 16-week old protein-restricted offspring had higher expression of Adrbk1, Bbs1, Dnmt3a, Gpr22, Inppl1, and Oxct1 genes. CONCLUSION: Gestational protein restriction leads to offspring low birth weight, increased systolic blood pressure and morphological heart alterations that could be related to early heart miRNA expression changes that perpetuate into adulthood and which are associated with the regulation of essential genes involved in cardiovascular development, heart morphology, function, and metabolism.
PREPRINT-CONTENT 0 Reads 0 Citations Effect of intracerebroventricular epinephrine microinjection on blood pressure and urinary sodium handling in gestationa... Jose Ar Gontijo, Patricia A Boer, Augusto H Custódio, Barbar... Published: 12 September 2018
bioRxiv, doi: 10.1101/415778
DOI See at publisher website
XXV Congresso de Iniciação Cientifica da Unicamp, doi: 10.19146/pibic-2017-77852
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BOOK-CHAPTER 0 Reads 0 Citations Effects of Maternal Protein Restriction on Nephrogenesis and Adult and Aging Kidney Patrícia Aline Boer, Ana Tereza Barufi Franco, José Antonio ... Published: 15 October 2017
Diet, Nutrition, and Fetal Programming, doi: 10.1007/978-3-319-60289-9_11
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BOOK-CHAPTER 0 Reads 0 Citations Maternal Protein Restriction and Effects on Behavior and Memory in Offspring Agnes Da Silva Lopes Oliveira, José Antonio Rocha Gontijo, P... Published: 15 October 2017
Diet, Nutrition, and Fetal Programming, doi: 10.1007/978-3-319-60289-9_12
DOI See at publisher website