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Bernd Kammerer  - - - 
Top co-authors See all
Arthur Gessler

87 shared publications

Eidgenossische Forschungsanstalt fur Wald Schnee und Landschaft

Nils Wiedemann

55 shared publications

Institute of Biochemistry and Molecular Biology, Centre for Biochemistry and Molecular Cell Research (ZBMZ), Faculty of Medicine, University of Freiburg, D-79104 Freiburg, Germany

L. Heide

54 shared publications

Pharmaceutical Institute, Eberhard Karls-University Tübingen, Tübingen, Germany

Carsten Gründemann

38 shared publications

Institute for Infection Prevention and Hospital Epidemiology, Center for Complementary Medicine, Faculty of Medicine, University of Freiburg, Freiburg, Germany, Freiburg, Germany

Roman Huber

27 shared publications

Center for Complementary Medicine, Institute for Infection Prevention and Hospital Epidemiology, Faculty of Medicine, University of Freiburg, Freiburg, Germany

Publication Record
Distribution of Articles published per year 
(2007 - 2018)
Total number of journals
published in
Publications See all
Article 2 Reads 0 Citations Metabolic profiling of isolated mitochondria and cytoplasm reveals compartment-specific metabolic responses Daqiang Pan, Caroline Lindau, Simon Lagies, Nils Wiedemann, ... Published: 31 March 2018
Metabolomics, doi: 10.1007/s11306-018-1352-x
DOI See at publisher website
ABS Show/hide abstract
Subcellular compartmentalization enables eukaryotic cells to carry out different reactions at the same time, resulting in different metabolite pools in the subcellular compartments. Thus, mutations affecting the mitochondrial energy metabolism could cause different metabolic alterations in mitochondria compared to the cytoplasm. Given that the metabolite pool in the cytosol is larger than that of other subcellular compartments, metabolic profiling of total cells could miss these compartment-specific metabolic alterations. To reveal compartment-specific metabolic differences, mitochondria and the cytoplasmic fraction of baker’s yeast Saccharomyces cerevisiae were isolated and subjected to metabolic profiling. Mitochondria were isolated through differential centrifugation and were analyzed together with the remaining cytoplasm by gas chromatography–mass spectrometry (GC–MS) based metabolic profiling. Seventy-two metabolites were identified, of which eight were found exclusively in mitochondria and sixteen exclusively in the cytoplasm. Based on the metabolic signature of mitochondria and of the cytoplasm, mutants of the succinate dehydrogenase (respiratory chain complex II) and of the FOF1-ATP-synthase (complex V) can be discriminated in both compartments by principal component analysis from wild-type and each other. These mitochondrial oxidative phosphorylation machinery mutants altered not only citric acid cycle related metabolites but also amino acids, fatty acids, purine and pyrimidine intermediates and others. By applying metabolomics to isolated mitochondria and the corresponding cytoplasm, compartment-specific metabolic signatures can be identified. This subcellular metabolomics analysis is a powerful tool to study the molecular mechanism of compartment-specific metabolic homeostasis in response to mutations affecting the mitochondrial metabolism.
Article 2 Reads 0 Citations Rosemary has immunosuppressant activity mediated through the STAT3 pathway Charlotte Von Schönfeld, Roman Huber, Rainer Trittler, Bernd... Published: 01 March 2018
Complementary Therapies in Medicine, doi: 10.1016/j.ctim.2018.03.004
DOI See at publisher website
Article 3 Reads 0 Citations Metabolic characterization of directly reprogrammed renal tubular epithelial cells (iRECs) Simon Lagies, Roman Pichler, Michael M. Kaminski, Manuel Sch... Published: 01 March 2018
Scientific Reports, doi: 10.1038/s41598-018-22073-7
DOI See at publisher website
Article 2 Reads 2 Citations Drought effects on root and needle terpenoid content of a coastal and an interior Douglas fir provenance Anita Kleiber, Qiuxiao Duan, Kirstin Jansen, Laura Verena Ju... Published: 10 October 2017
Tree Physiology, doi: 10.1093/treephys/tpx113
DOI See at publisher website
Article 2 Reads 6 Citations Alterations of the exo- and endometabolite profiles in breast cancer cell lines: A mass spectrometry-based metabolomics ... Lucas Willmann, Manuel Schlimpert, Marc Hirschfeld, Thalia E... Published: 01 June 2016
Analytica Chimica Acta, doi: 10.1016/j.aca.2016.04.047
DOI See at publisher website
PubMed View at PubMed
Article 2 Reads 4 Citations Metabolome analysis via comprehensive two-dimensional liquid chromatography: identification of modified nucleosides from... Lucas Willmann, Thalia Erbes, Sonja Krieger, Jens Trafkowski... Published: 04 March 2015
Zeitschrift f�r Analytische Chemie, doi: 10.1007/s00216-015-8516-6
DOI See at publisher website
PubMed View at PubMed