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Bernd Kammerer  - - - 
Top co-authors See all
Arthur Gessler

86 shared publications

Nils Wiedemann

58 shared publications

Lutz Heide

52 shared publications

Pharmazeutisches Institut, Eberhard-Karls-Universität Tübingen, Auf der Morgenstelle 8, 72076 Tübingen, Germany

Carsten Gründemann

39 shared publications

Roman Huber

30 shared publications

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Publication Record
Distribution of Articles published per year 
(2007 - 2018)
Total number of journals
published in
 
9
 
Publications See all
Article 0 Reads 0 Citations Metabolic profiling of isolated mitochondria and cytoplasm reveals compartment-specific metabolic responses Daqiang Pan, Caroline Lindau, Simon Lagies, Nils Wiedemann, ... Published: 31 March 2018
Metabolomics, doi: 10.1007/s11306-018-1352-x
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Subcellular compartmentalization enables eukaryotic cells to carry out different reactions at the same time, resulting in different metabolite pools in the subcellular compartments. Thus, mutations affecting the mitochondrial energy metabolism could cause different metabolic alterations in mitochondria compared to the cytoplasm. Given that the metabolite pool in the cytosol is larger than that of other subcellular compartments, metabolic profiling of total cells could miss these compartment-specific metabolic alterations. To reveal compartment-specific metabolic differences, mitochondria and the cytoplasmic fraction of baker’s yeast Saccharomyces cerevisiae were isolated and subjected to metabolic profiling. Mitochondria were isolated through differential centrifugation and were analyzed together with the remaining cytoplasm by gas chromatography–mass spectrometry (GC–MS) based metabolic profiling. Seventy-two metabolites were identified, of which eight were found exclusively in mitochondria and sixteen exclusively in the cytoplasm. Based on the metabolic signature of mitochondria and of the cytoplasm, mutants of the succinate dehydrogenase (respiratory chain complex II) and of the FOF1-ATP-synthase (complex V) can be discriminated in both compartments by principal component analysis from wild-type and each other. These mitochondrial oxidative phosphorylation machinery mutants altered not only citric acid cycle related metabolites but also amino acids, fatty acids, purine and pyrimidine intermediates and others. By applying metabolomics to isolated mitochondria and the corresponding cytoplasm, compartment-specific metabolic signatures can be identified. This subcellular metabolomics analysis is a powerful tool to study the molecular mechanism of compartment-specific metabolic homeostasis in response to mutations affecting the mitochondrial metabolism.
Article 1 Read 0 Citations Rosemary has immunosuppressant activity mediated through the STAT3 pathway Charlotte Von Schönfeld, Roman Huber, Rainer Trittler, Bernd... Published: 01 March 2018
Complementary Therapies in Medicine, doi: 10.1016/j.ctim.2018.03.004
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In Europe extracts of Rosmarinus officinalis were traditionally used for the treatment of rheumatic diseases. We investigated the capacity of standardized aqueous extracts of Rosmarinus officinalis on human primary lymphocyte function in vitro, as activated lymphocytes are an important mediator of rheumatic diseases. Lymphocyte proliferation was measured using membrane-permeable dye carboxyfluorescein diacetate succinimidyl ester (CFSE). Apoptosis was analysed by surface staining of phosphatidylserine (annexin V-assay) and necrosis was analysed by staining with propidium iodide. Modification of cell activity was detected by surface staining of CD69 and CD25. The activity of STAT3 in T-lymphocytes was determined by intracellular staining of STAT3 molecules. The Rosmarinus officinalis extract was investigated at concentrations of 0.05–25 mg/mL. Analysis of the extract was performed using HPLC methods. Rosmarinus officinalis inhibited proliferation of human lymphocytes and CD4+ T-cells in a dose-dependent manner (3.1–25 mg/mL) through induction of apoptosis. The intracellular signalling pathway STAT3 in T-cells, but not NF-kappaB and ERK1/2 in T- and B-cells was inhibited in a dose-dependent manner by Rosmarinus officinalis (0.2-6.2 mg/mL). Rosmanol, carnosolic acid, carnosol and trans-caffeic acid were tested in the same cellular models as the crude extract. From these, only trans-caffeic acid inhibited lymphocyte proliferation and STAT3 (30–100 μg/mL). Trans-caffeic acid was found in the extract in a concentration of 14.7 μg/mL. We conclude that an immunosuppressive effect of Rosmarinus officinalis is mostly due to the effect of trans-caffeic acid. It results in inhibition of the activity of STAT3 causing induction of apoptosis and inhibition of proliferation of T-lymphocytes.
Article 1 Read 0 Citations Metabolic characterization of directly reprogrammed renal tubular epithelial cells (iRECs) Simon Lagies, Roman Pichler, Michael M. Kaminski, Manuel Sch... Published: 01 March 2018
Scientific Reports, doi: 10.1038/s41598-018-22073-7
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Article 1 Read 0 Citations Drought effects on root and needle terpenoid content of a coastal and an interior Douglas fir provenance Anita Kleiber, Qiuxiao Duan, Kirstin Jansen, Laura Verena Ju... Published: 10 October 2017
Tree Physiology, doi: 10.1093/treephys/tpx113
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Article 0 Reads 6 Citations Alterations of the exo- and endometabolite profiles in breast cancer cell lines: a mass spectrometry-based metabolomics ... Lucas Willmann, Manuel Schlimpert, Marc Hirschfeld, Thalia E... Published: 01 June 2016
Analytica Chimica Acta, doi: 10.1016/j.aca.2016.04.047
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Highlights•Different extraction procedures have been applied for exo- and endometabolome.•Cell line analysis was performed by two different analytical platforms: untargeted LC-Q-TOF MS as well as targeted LC-QqQ MS.•An UPLC-ESI-Q-TOF method based on a scheduled precursor list was established for discrimination of cell lines.•Significant differences between breast cancer and breast epithelial cell lines are promising for diagnosis and sub classification of breast cancer. AbstractIn recent years, knowledge about metabolite changes which are characteristic for the physiologic state of cancer cells has been acquired by liquid chromatography coupled to mass spectrometry. Distinct molecularly characterized breast cancer cell lines provide an unbiased and standardized in vitro tumor model reflecting the heterogeneity of the disease. Tandem mass spectrometry is a widely applied analytical platform and highly sensitive technique for analysis of complex biological samples. Endo- and exometabolite analysis of the breast cancer cell lines MDA-MB-231, -453 and BT-474 as well as the breast epithelial cell line MCF-10A has been performed using two different analytical platforms: UPLC-ESI-Q-TOF based on a scheduled precursor list has been applied for highlighting of significant differences between cell lines and HPLC-ESI-QqQ using multiple reaction monitoring has been utilized for a targeted approach focusing on RNA metabolism and interconnected pathways, respectively. Statistical analysis enabled a clear discrimination of the breast epithelial from the breast cancer cell lines. As an effect of oxidative stress, a decreased GSH/GSSG ratio has been detected in breast cancer cell lines. The triple negative breast cancer cell line MDA-MB-231 showed an elevation in nicotinamide, 1-ribosyl-nicotinamide and NAD+ reflecting the increased energy demand in triple negative breast cancer, which has a more aggressive clinical course than other forms of breast cancer. Obtained distinct metabolite pattern could be correlated with distinct molecular characteristics of breast cancer cells. Results and methodology of this preliminary in vitro study could be transferred to in vivo studies with breast cancer patients. Graphical abstract
Article 0 Reads 3 Citations Metabolome analysis via comprehensive two-dimensional liquid chromatography: identification of modified nucleosides from... Lucas Willmann, Thalia Erbes, Sonja Krieger, Jens Trafkowski... Published: 04 March 2015
Analytical and Bioanalytical Chemistry, doi: 10.1007/s00216-015-8516-6
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Modified nucleosides derived from the RNA metabolism constitute an important chemical class, which are discussed as potential biomarkers in the detection of mammalian breast cancer. Not only the variability of modifications, but also the complexity of biological matrices such as urinary samples poses challenges in the analysis of modified nucleosides. In the present work, a comprehensive two-dimensional liquid chromatography mass spectrometry (2D-LC-MS) approach for the analysis of modified nucleosides in biological samples was established. For prepurification of urinary samples and cell culture supernatants, we performed a cis-diol specific affinity chromatography using boronate-derivatized polyacrylamide gel. In order to establish a 2D-LC method, we tested numerous column combinations and chromatographic conditions. In order to determine the target compounds, we coupled the 2D-LC setup to a triple quadrupole mass spectrometer performing full scans, neutral loss scans, and multiple reaction monitoring (MRM). The combination of a Zorbax Eclipse Plus C18 column with a Zorbax Bonus-RP column was found to deliver a high degree of orthogonality and adequate separation. By application of 2D-LC-MS approaches, we were able to detect 28 target compounds from RNA metabolism and crosslinked pathways in urinary samples and 26 target compounds in cell culture supernatants, respectively. This is the first demonstration of the applicability and benefit of 2D-LC-MS for the targeted metabolome analysis of modified nucleosides and compounds from crosslinked pathways in different biological matrices.