Bioceramics are extensively used in bone tissue engineering, promoting tissue bonding, bone growth, and damage repair. Moreover, some bioceramics enhance the efficacy of skin healing by regulating the activity of various cell types (https://doi.org/10.1016/j.biomaterials.2022.121652). Given the absence of previous experimental studies, this work could contribute to the advancement of scientific knowledge in the area of ​​biomaterials. So, the objective of this study was to characterize and evaluate bioceramic powder samples with regard to their potential use in bone and skin regeneration. An optical microscopic analysis revealed that the morphology and size of the different bioceramic samples exhibited irregularities. The smallest particles were observed in the B2P, BG-2A, BG-2B, E1, F1, and G1 groups. The commercial 45S5 exhibited fine, pointed particles, whereas the B1P group had particles exceeding 100 μm in length. The E1 group exhibited the most negative zeta potential value, indicating that it was the most stable sample. In contrast, the B2P, BG-2A, BG-2B, F1, and G1 groups exhibited zeta potential values close to -25 mV, indicating that they exhibited moderate colloidal stability in suspension. Conversely, the B1P and 45S5 groups exhibited the least stable characteristics. An assessment of mitochondrial metabolism carried out on the MRC-5 fibroblast cell line exposed to the bioceramics using the MTT assay (n = 4 replicates/group) revealed that there was no significant impact on cell viability after 24 hours. These findings indicate that these new bioceramics possess favorable physicochemical properties and are not cytotoxic. The future prospects include the incorporation of bioceramics in bioinks for bone and skin regeneration.

Support: The Office of Naval Research Global (ONRG Award N62909-21-1-2026); National Institute of Science and Technology for Regenerative Medicine (INCT-Regenera); and Stem Cell Research Institute (IPCT).