INTRODUCTION
In ancient times, silver played an important role as a novel metal ion in curing many diseases and fighting against many infections. Currently, silver is used in the form of AgNPs for targeting many biomedical and physio-chemical reactions to fulfill research goals .But many drawbacks are also reported in AgNP reactions, such as allergy and environmental risks. Therefore, to avoid all these side-effects, plant-based AgNPs are synthesized. In our research, we have used silver nano-particles of Phyllanthus emblica and Adhatoda vasica leaf extract and carried out a comparative study on microbes.
METHODS
Leaves were first collected and then crushed into a powder. Next, we made a water-based extract solution by heating the mixture to 80 degrees Celsius for three to four hours using a magnetic stirrer. Finally, the leaf extract was combined with 1M silver nitrate solution, which was made by dissolving 1.7 grams of silver nitrate in 100 milliliters of water. Finally, the mixture of amla and adusa silver nitrate was centrifuged at 12000 rpm for 30 minutes, discarding the supernatant and collecting the dark pellet to form AgNPs of leaf extract. Finally, the leaf extract was collected in the form of a powder and dried for two to three days in a dark place. Using the disc diffusion and well diffusion methods, we investigated the effects of these AgNP powders at varying concentrations against bacteria that cause disease, such as E. Coli, S. Aureus, Mucor, and Aspergillus strains. Additionally, we utilized the commercial antibiotic streptomycin to complete a comparative study.
CONCLUSIONS
Secondary metabolites in plant leaves make plant-based drug systems and AgNP molecules more effective and eco-friendly compared to chemical-based AgNPs. In our research, a comparative study of the effects of amla and adusa leaf extract AgNPs on microbes produced positive results compared to the commercial antibiotic streptomycin, with their ability to kill microbes clearly shown by the formation of a zone of inhibition on the Petri dishes.
