Introduction: Sourdough bread is made through the fermentation of dough using naturally occurring lactobacilli and wild yeast, unlike common bread, which uses commercial baker's yeast. It utilizes a starter culture of flour and water that captures wild yeast and bacteria, giving it a distinctive tangy flavor and chewy texture. Nutritionally, sourdough offers several advantages: the fermentation process breaks down gluten, making it easier to digest for those with gluten sensitivities. Additionally, lactic acid bacteria improve nutrient bioavailability, and the lower glycemic index of sourdough can help maintain stable blood sugar levels.

Methods: To investigate the influence of adding selected carbohydrates (beet sugar, honey, active malt flour) on sourdough bread's parameters, a modified three-stage sourdough management method was chosen. The dough, consisting of 65% rye and 35% wheat flour, was kneaded for 5 minutes for optimal ingredient homogenization and gluten development. It was then fermented at 28 °C for 160 minutes. The content of organic acids in the dough was measured using ion chromatography, and the increase in dough volume and pH was monitored. Oval loaves were baked from 350 g pieces of dough. Each loaf was cut transversely, and the cut from its middle was measured for resistance to pressure applied at right angles using a Brookfield LFRA 1500 texturometer.

Results: According to the analysis, sourdough fortification with honey tends to produce the maximal volume for the loaf and the most tender crumb at the same time. Higher additions of beet sugar increase the total acidity of the dough the most significantly. In contrast, malt flour provides a smooth, sweetish aroma to the bread. The addition of malt flour causes the inhibition of acetic acid production during sourdough fermentation. Unfortunately, a lower concentration of acetate and a higher content of remaining sugars at the same time shortened the microbial shelf-life of the bread after baking.