Recent advancements in 3D bioprinting technology have shown significant promise in developing innovative treatments for pediatric burn victims. This systematic literature analysis examines the progress in isolating and expanding autologous skin cells, specifically keratinocytes and fibroblasts, from pediatric patients for use in bioprinting applications. Key studies demonstrate the ability to effectively culture these cells, which are crucial for generating viable skin grafts. The systematic review also explores the evolution of bio-inks and the optimization of 3D bioprinting protocols aimed at creating multi-layered skin constructs that closely mimic natural skin architecture. Preclinical studies have been analyzed to evaluate the integration, functionality, and cosmetic outcomes of bioprinted skin grafts in animal models, highlighting their potential to improve healing and reduce scarring. Additionally, the review addresses the challenges of translating these promising preclinical findings into clinical trials, considering factors such as biocompatibility, immune response, and the scalability of bioprinting techniques. This comprehensive review underscores the transformative potential of 3D bioprinting in pediatric burn treatment, while also identifying the critical areas where further research and development are needed to bring these advancements to clinical practice. To fully realize the clinical application of 3D bioprinting for pediatric burn victims, interdisciplinary collaboration and long-term studies are essential in addressing the complex ethical, regulatory, and technical challenges associated with this cutting-edge technology.