Subunit vaccines are safer than live-attenuated or inactivated vaccines. However, their limited immunogenicity and susceptibility to metabolic degradation require strong adjuvants and/or conjugation to delivery systems to induce a robust, antigen-specific immune response. Interestingly, synthetic peptides can be useful and polyvalent building blocks for development of self-adjuvanted nanoparticles. Recent studies showed that amphiphilic peptides can increase antigen density, promote cellular uptake by APCs and activate T and B lymphocytes. Additionally, short peptide monomers forming cross-β-sheet fibrils such as I₁₀ have shown potential in inducing strong immunity against the grafted epitope. However, direct comparison of the intrinsic immunogenicity of peptide amphiphilic cylindrical micelles and cross-β peptide fibrils has never been performed. In this work, the amphiphilic peptide C₁₆-V₃A₃K₃(PA) and the I₁₀ β-peptide were each linked to two epitope models, OVA_253-266 and OVA_323-339, able to polarize the resulting adaptive immune response differently. Biophysical analysis showed that both nanoplatforms formed β-sheet-rich nanofilament structures with epitopes exposed on their surfaces. Intramuscular administration in mice led to a strong, antigen-specific humoral response without additional adjuvants. This study illuminates the potential of synthetic self-assembling nanoplatforms as universal antigen carriers, enabling the rapid development of vaccines to combat infectious diseases.