Introduction. Nanocrystalline cellulose (NCC) has unique physico-chemical properties and is considered an effective substitute for microcrystalline cellulose (E460i). However, the use of NCC in food production is hindered by a lack of knowledge about the risks of its effects on the human body during prolonged consumption with food.

Methods. The potential neurotropic properties of NCC were studied using a complex of neurophysiological methods (CRPA, EPM) with the daily consumption of NCC by Wistar rats at doses of 1-100 mg/kg b.w. for 56 days.

Results. The consumption of NCC in the entire dose range had an effect on the motor activity of animals in the EPM. In animals that consumed NCC at a dose of 100 mg/ kg b.w., a whole range of significant changes in behavioral reactions were revealed compared with the control, among which there were signs of anxiety-like behavior. As shown by an analysis of the movement of rats around the maze, there was an almost threefold increase in the ratio of stay in the open and closed arms of the maze. During the development of a conditioned reflex in the CRPA installation, rats that consumed NCC in the highest dose were characterized by a significant decrease in latency time before entering the dark compartment of the installation. There was no significant effect of NCC on the indicators of short-term and long-term memory.

Conclusion. Dietary intake of NCC, especially in high doses of 10-100 mg/kg b.w., is accompanied by neurotropic effects in rats. The NOAEL of NCC for a 56-day intake with a diet is, according to the study of behavioral indicators, in any case, less than 1 mg/kg b.w.

Funding. Ministry of Education and Science of the Russian Federation (FGMF-2025-0004).