Stingless bee honey is a functional food increasingly valued for its distinctive taste and diverse health-promoting properties. Although its antioxidant activity is well documented, the influence of the climatic conditions and bee species on its bioactive properties remains underexplored. This study investigated the effects of seasonal variation and the bee species on the antioxidant capacity of stingless bee honey under a tropical climate. Honey samples were collected throughout the year, during both the dry and rainy seasons. A comprehensive set of antioxidant assays was employed, including assays for the total phenolic content, ABTS, DPPH, superoxide anion and nitric oxide radical scavenging activities, iron-chelating activity, and ferric reducing antioxidant power (FRAP). Honey samples collected during the dry season exhibited a higher overall antioxidant capacity than those from the rainy season. Despite this trend, statistical analysis revealed no significant differences between the seasons, likely due to the consistently warm and humid conditions provided by the tropical climate. In contrast, the bee species showed a notable impact. Chaste tree honey produced by Heterotrigona itama showed significantly greater nitric oxide scavenging activity (365.31 ± 64.62 %) and FRAP (1.90 ± 0.39 mmol Fe (II)/ kg) than honey with the same botanical origin produced by Geniotrigona thoracica (276.55 ± 61.39 %; 1.47 ± 0.43 mmol Fe (II)/ kg), highlighting the considerable influence of the bee species on the antioxidant potential. These findings suggest that the stingless bee species contributes more substantially to the antioxidant profile than seasonal variation in tropical climates. These findings offer valuable insights for the industry into optimizing stingless bee honey production, particularly improving its nutritional and functional value as a health-enhancing food product.