ABSTRACT:

Introduction: Hyperlipidemia is abnormal of lipid metabolism that presents in the bloodstream. The study aims is to investigate the effects on a combination mixture of Zinger officinale, Allium sativum, Citrus lemon, honey, and Malus domestica vinegar (ZACA) in rats fed with high cholesterol diet (HCD). Method: 36 male Sprague dawley rats were divided into 6 groups. ZACA extracts (1mg/kg, 3 mg/kg, 5 mg/kg of bodyweight) were administered along with high cholesterol diet (HCD) for 18 weeks. Simvastatin 10 mg/kg of bodyweight was used as a control. Results: In vitro; ZACA extracts had oxygen radical absorbance capacity (ORAC) of 2000 µmol TE/100 mL, total phenolic content (TPC) is 7537 ± 54.5%, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity is 27.34 ± 2.71% , Elastase inhibitory assay is (29.29 ± 1.65% and lipoxygenase inhibitory assay is 98.58 ± 1.42%. The high performance liquid chromatography (HPLC) detected the presence of Hesperidin in ZACA extract that acts as anti-oxidative and anti-inflammatory agent. In vivo; ZACA extract decreased bodyweight, adipose tissue weight, and improved lipid profiles (total cholesterol, triglycerides, low density lipoprotein, and high density lipoprotein). Conclusion: The results demonstrate that ZACA extracts have a protective effect against hyperlipidemia and beneficial as dietary supplement.