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An assessment of applicability of Illumina GoatSNP50 BeadChip for genetic studies of Caucaian tur (Capra caucasica) †
* 1 , 1 , 2 , 1 , 3 , 3 , 1, 4 , 1
1  L.K. Ernst Federal Science Center for Animal Husbandry, Podolsk, Russia
2  Department of game management and bioecology, Irkutsk State University of Agriculture, 664038 Irkutsk region, Russia
3  Institute of Genome Biology, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany
4  Institute of Animal Breeding and Genetics, University of Veterinary Medicine (VMU), Vienna, Austria

Abstract:

Caucasian tur (Capra caucasica) is native to Greater Caucasus Mountain Chain from Azerbaijan and Georgia in the East to Krasnodar region of Russia in the West. This species is divided into two subspecies – East-Caucasian tur and West-Caucasian tur and an admixed subpopulation referred to as Mid-Caucasian tur. Up to date most of genetic studies of Caucasian tur were based on mtDNA sequences and comprehensive investigation of nuclear DNA is required for clarification of its genetic diversity and population structure. In our work, we assessed applicability of Illumina GoatSNP50 BeadChip for genetic studies of Caucasian tur. Fifteen specimens of Capra caucasica including East Caucasian tur from Dagestan (E_TUR, n=5), West Caucasian tur from Karachay-Cherkessia (W_TUR, n=5) and Mid-Caucasian tur from Kabardino-Balkaria (M_TUR, n=5) were genotyped. After quality control, 4758 polymorphic loci, which were distributed all over 29 autosomes, were detected. The lowest number of SNPs was found on the 25th chromosome – 68, and the highest on the 1st chromosome – 348. It was shown that all the three groups of Caucasian tur clustered separately. A total of 2061 SNPs were common for all the subpopulations, 594 were found only in W_TUR, 689 in E_TUR and 530 in M_TUR. Individual heterozygosity ranged from 0.309 to 0.319 in W_TUR, from 0.244 to 0.278 in E_TUR and from 0.288 to 0.323 in M_TUR. Thus in our study we demonstrated that the Illumina GoatSNP50 BeadChip designed for domestic goats can be used as useful tool for genetic studies of Caucasian tur.

Keywords: wild goats; single nucleotide polymorphisms; genetic diversity; population structure
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