Introduction. Staphylococcus aureus is an opportunistic pathogen of clinical importance, it can cause mild infections to death. The widespread use of antibiotics, particularly their inappropriate and excessive use, has favored the emergence and maintenance of strains of S. aureus resistant to multiple antibiotics such as penicillin or methicillin (methicillin-resistant S. aureus (MRSA) that are associated with high rates of morbidity and mortality in many regions of the world. The aim of this work was to determine the prevalence of MRSA strains in children from Mexico City. Methods. 476 pharyngeal and nasal exudates were performed on pediatric patients under 16 years of age between 2013 and 2019, they were inoculated on Mannitol Salt Agar and incubated for 24 hours at 37 °C. The presence of S. aureus was determined by fermentation of mannitol and positivity to coagulase. The minimum inhibitory concentration (MIC) for oxacillin were also performed. Methicillin resistant strains were those that grew in concentrations greater than 4 µg/mL. Results. A total of 240 women (50.42%) and 236 men (49.58%) (N = 476) with an average age of 7.39 years (+/- 2.76) were studied, in which 230 strains of S. aureus in the pharynx were isolated (48.31%), and 210 in nose (44.11%). Most isolated S. aureus strains are methicillin-sensitive (205 strains isolated from the pharynx and 176 strains from the nose), only 25 MRSA strains were isolated from the pharynx (5.25%) and 34 MRSA strains from the nose (7.14%). In addition, 137 carriers in both sites (28.78%), 93 exclusive carriers in the pharynx (19.53%) and 73 exclusive carriers in the nose (15.33%), and 246 non-carriers of S. aureus were found in both sites (51.68%). Conclusions. A greater number of S. aureus carriers were found in the pharynx than in the nose, the prevalence of MRSA strains is low, although most of the MRSA strains were found in the nose.