Events The 1st International Electronic Conference on Chemical Sensors and Analytical Chemistry

Event submissions

Published

10.3390/CSAC2021-10430 (registering DOI)

This submission belongs to the session B. Biosensors of the event The 1st International Electronic Conference on Chemical Sensors and Analytical Chemistry

Published date

30 Jun, 2021

Academic Editor

Run Zhang

Citation

Zhenzhong Guo, Dan Tao, Nicole Jaffrezic-Renault, An ultrasensitive aptasensor for the analysis of Aβ oligomers in serum, in Proceedings of The 1st International Electronic Conference on Chemical Sensors and Analytical Chemistry, 1 July–15 July 2021, MDPI: Basel, Switzerland, doi: 10.3390/CSAC2021-10430

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An ultrasensitive aptasensor for the analysis of Aβ oligomers in serum

Dan Tao ^1,2,3

Nicole Jaffrezic-Renault ⁴

Zhenzhong Guo ⁵

1. Hubei Province Key Laboratory of Occupational Hazard identification and Control, Wuhan University of Science and Technology, Wuhan 430065, PR China

2. School of Public Health, Medical College, Wuhan University of Science and Technology, Wuhan 430065, PR China

3. Resources and Environmental Engineering College, Wuhan University of Science and Technology, Wuhan 430081, China

4. University of Lyon, Institute of Analytical Sciences, UMR-CNRS 5280, 5, La Doua Street, Villeurbanne 69100, France

5. Hubei Province Key Laboratory of Occupational Hazard identification and Control, Wuhan University of Science and Technology, Wuhan 430065, PR China, China

Abstract

It is one of the great challenges to improve the sensitivity for early detection of biomarker in Alzheimer's disease (AD). In this work, an electrochemical aptasensor was prepared based on thionine (Th) - functionalized three - dimensional carbon nanomaterials (reduced graphene oxide (rGO) and multi-walled carbon nanotubes (MWCNTs) immobilized aptamer for the analysis of Aβ oligomers (AβO), one of the AD biomarkers. Th, the positively charged planar aromatic molecule, could form a large number of π - conjugated structures with rGO and MWCNTs, thereby bringing extra stability to the conjugated macromolecules and improve the structural stability and capacitive properties of Th-rGO-MWCNTs nanocomposites. Under the optimal conditions, DPV response decreased with the increase of AβO concentration. The aptasensor has a good linear range of 0.0443 pM to 443.00 pM with a LOD of 0.01 pM. It also has excellent stability and selectivity for AβO detection. This aptasensor thus has a great potential for early diagnosis of AD.

Keywords

Aβ oligomers

Alzheimer's disease

Aptasensor

Th-rGO-MWCNTs nanocomposites

human serum

Manuscript

sciforum-046133.pdf

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