Investigation of Selected Piperazine-2,5-diones on Their Effect on Cartilage-Related Cells

Various chronic inflammatory diseases have become a problem, especially in the Western world. Whether it is inflammation of visceral organs, joints, bones, etc., it is always a physiological reaction of the body, which always tries to eradicate harmful substances and restore tissue homeostasis. Unfortunately, prolonged or chronic inflammation often results in damage to the affected tissues. Cartilage damage, diseases such as osteoarthritis, rheumatoid arthritis and arthrosis, are very common. In addition to suppressing inflammation in the joints and around the cartilage, it is advantageous to administer compounds that are capable of stimulating cartilage growth and regenerate damaged tissue. Variously substituted piperazine-2,5-dione derivatives were investigated as compounds with a potential effect on cartilage regeneration. A series of assays were performed to evaluate their cytotoxicity, anti-inflammatory activity, ability to potentiate chondrocyte proliferation, and suppress synovial cell growth. The compounds proved to be completely non-toxic for all used types of cells up to the concentration of 20 µM. Unfortunately, their evaluated biological activity proved to be insignificant in comparison with untreated cells.