Fusarium wilt, caused by Fusarium oxysporum f.sp. cubense Tropical Race 4 (Foc TR4) is one of the most severe banana diseases in the world. In this study, the endophytic bacteria Kocuria rhizophila which had been isolated previously from banana leaves was used. Endophytic bacteria are proven to increase plant growth and can trigger plant systemic resistance (ISR). The biochemical response of bananas inoculated with K. rhizophila is unknown, so this study aimed to explore the activity of peroxidase (PO), polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL) enzymes, and analyze the total phenolic content of banana plants inoculated with K. rhizophila before being infected with Foc TR4. Banana plants treated with K. rhizophila showed increased PO enzyme activity increased and reached its highest activity 72 hours after inoculation in shoots (0,1640±0,0335