The Phenotypic Reactivity of Passiflora incarnata L. on Various Content of Mineral Salts and Regulators During Micropropagation and Acclimatization

Passiflora incarnata is ornamental and medicinal plant that contains a valuable active chemical derivatives of apigenin and luteolin. Conventional cultivation of this plant in Poland is a very problematic, caused by the low percentage of seed germination, viability of seedlings, and plant diseases which can seriously reduce the productivity of P. incarnata. An alternative and promising way to solve these problems may be used the technique of micropropagation, which may have applied for the plant multiplication under controlled conditions and have offered the production of healthy, pathogen-free and true-to-type plants.

The aim of this study was to detrmine 1) the influence of IAA (0.1-1.0 mg L^-1), and IBA (0.1-1.0 mg L^-1) on brasilian seed germination, and 2) the influence of various concetrations of mineral salts in Murashige and Shoog (MS), Gamborg (B-5), Shenk-Hildebrandt (SH) and Phytamax media on growth, development and condition of plant in vitro, 3) induction of adventitious shoots using nodal fragments under influence of BAP (0.1-1.0 mg L^-1), TDZ (0.1-1.0 mg L^-1), KIN (0.1-1.0 mg L^-1) with IAA (0.1 mg L^-1). Results showed that 1) MS medium with IAA (1.0 mg L^-1) has been most efective in induction of seed germination (60%); 2) Gamborg (B-5) medium has been more favorable for plant growth and development, and 3) SH with BAP (1.0 mg L^-1) and TDZ (0.1 L^-1) with IAA induced more adventitious buds and new regenerated plantlets. After rooting, 100% obtrained plants have been actimatizared to ex vitro conditions and have been observed in greenhaouse.