EventsThe 1st International Electronic Conference on Processes: Processes System Innovation
Event submissions
Published
with-doi10.3390/ECP2022-12612 (registering DOI)
This submission belongs to the session 5. Biological Processes and Systems of the event The 1st International Electronic Conference on Processes: Processes System Innovation
Published date
17 May, 2022
Academic Editor
author-avatarfrancesca spyrakis
Citation
DJEHICHE Cheima, arrar lekhmici, benzidane nadia, type ll collagen purified from chicken cartilage, in Proceedings of The 1st International Electronic Conference on Processes: Processes System Innovation, 17 May–31 May 2022, MDPI: Basel, Switzerland, doi: 10.3390/ECP2022-12612
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type ll collagen purified from chicken cartilage

DJEHICHE Cheima 1
arrar lekhmici 2
benzidane nadia 3
1. PhD student
2. professor
3. MCA
Abstract

The study presented in this work focused on the extraction and purification of type II collagen using an acid-enzymatic extraction starting with cartilage digestion using pepsin and acetic acid followed by precipitation using Nacl . collagen solubilized and recovered by acetic acid.

The extract obtained is purified by electrophoresis gives an alpha chain and its dimers of the betta chain on SDS PAGE so the analysis by scanning 200 to 400 nm on uv-visible spectrophotometer gives an absorbance at 225 nm.

The purified collagen from chicken sternum cartilage was type 2 collagen and could find applications in the induction of arthritis .

Keywords
collagen type ll
chicken cartilage
acid-enzymatic extraction
pepsin
precipitation
purification
electrophoresis
visible uv spectro.
Manuscript
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