EventsThe 2nd International Electronic Conference on Biomolecules
Published
This submission belongs to the session A. Biomacromolecules: Proteins of the event The 2nd International Electronic Conference on Biomolecules
Published date
09 Nov, 2022
Academic Editor
author-avatarVladimir Uversky
Citation
Mohd Hairul Mohd Salleh, Kwa Jia Sim, Aida Fitri Peli, Mohamad Syazwan Ngalimat, Mini Literature Review on Current Advances in Protein Purification Technique, in Proceedings of The 2nd International Electronic Conference on Biomolecules, 1 November–15 November 2022, MDPI: Basel, Switzerland, doi: 10.3390/IECBM2022-13507
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Mini Literature Review on Current Advances in Protein Purification Technique

Kwa Jia Sim 3
Aida Fitri Peli 4
image
1. Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400, UPM, Serdang, Selangor, Malaysia., Malaysia
2. Royal Malaysian Customs Department, Persiaran Perdana, Presint 2, 62596 Putrajaya, Malaysia.
3. Department of Industrial Biotechnology, Faculty of Biotechnology and Biomolecular Sciences, Persiaran Universiti, Universiti Putra Malaysia, 43400, UPM, Serdang, Selangor, Malaysia.
4. Department of Chemical and Environmental Engineering, Faculty of Engineering, Persiaran Universiti, Universiti Putra Malaysia, 43400, UPM, Serdang, Selangor, Malaysia.
5. Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Persiaran Universiti, Universiti Putra Malaysia, 43400, UPM, Serdang, Selangor, Malaysia.
Abstract

Protein purification is an ever-vital approach for each academia and industry. This paper mainly reviews and discusses one of the center contents of proteomics, the latest advances in separation technology for protein components focusing on five different methods. The MCPA system is an economically amazing invention for protein therapy researchers to purify samples. Because of the excessive protease interest of Ulp1 toward SUMO fusion, excessive protein products may be done inside 1/2 of an hour through this method. The magnetic separation strategies will offer a higher method of protein purification withinside the close to imminent because of a few advantages. The evaluation established that the ATPS technique became a cost-effective, time-saving (30 min), and high-recuperation approach that can be scaled up for commercial purposes. Herein, ATPS may be an ability approach for the purification of single-step separation and keep away from multistep purification just like the chromatography technique. Therefore, our recent reviews provide a capable technique for competent protein purification.

Keywords
MCPA system
SUMO fusion
magnetic separation
ATPS technique
chromatography technique.
Manuscript
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