Plant-derived ingredients almost contribute to 25% of prescription pharmaceuticals yet only a small percentage of the plants in the world have been assessed for their prospective pharmaceutical use. Due to an increasing demand for chemical diversity in screening programs, seeking therapeutic drugs from natural products, interest particularly in edible plants has grown throughout the world. Botanicals and herbal preparations for medicinal usage contain various types of bioactive compounds. The present work focused on the analytical methodologies, which include the phytochemical screening, extraction, isolation and characterization of bioactive compound from the G diversifolia methanolic leaf extract. One phyto-constituent Caffeic acid was isolated from Girardinia diversifolia. The analysis of bioactive compound present in the G diversifolia extract involving the applications of chromatographic techniques such as HPLC and, TLC as well as non-chromatographic techniques such as MS and Fourier Transform Infra-Red (FTIR).