Multivalent systems are biotechnological tools that utilize multiple, specific interactions to achieve a desired function. These systems often involve the use of multivalent ligands, which are molecules that can bind to multiple target molecules simultaneously, and multivalent receptors. By leveraging the power of multiple, specific interactions, multivalent systems can achieve higher levels of effectiveness and specificity than traditional monovalent approaches. Research on multivalency is currently an interplay of the fields of biochemistry and supramolecular chemistry. In biotechnology, multivalent systems have been used in a variety of applications, including drug delivery, protein engineering, and immune system modulation. The use of multivalent systems has the potential to revolutionize the way that biotechnology approaches complex problems and has already led to numerous breakthroughs in a variety of fields. In this review, we focus on the application of multivalent systems in biotechnology and their potential in nucleic acid therapies.