EventsHolography Meets Advanced Manufacturing
Published
with-doi10.3390/HMAM2-14150 (registering DOI)
This submission belongs to the session H1. Holography 1 of the event Holography Meets Advanced Manufacturing
Published date
13 Mar, 2023
Academic Editor
author-avatarVIJAYAKUMAR ANAND
Citation
Molong Han, Daniel Smith, Soon Hock Ng, Tomas Katkus, Aravind Simon John Francis Rajeswary, Periyasamy Angamuthu Praveen, Keith R Bambery, Mark J Tobin, Jitraporn Vongsvivut, Saulius Juodkazis, Vijayakumar Anand, Single Shot Lensless Interferenceless Phase Imaging of Biochemical Samples Using Synchrotron Near Infrared Beam, in Proceedings of Holography Meets Advanced Manufacturing, 20 February–22 February 2023, MDPI: Basel, Switzerland, doi: 10.3390/HMAM2-14150
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Single Shot Lensless Interferenceless Phase Imaging of Biochemical Samples Using Synchrotron Near Infrared Beam

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Mark J Tobin 4
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1. Optical Sciences Centre and ARC Training Centre in Surface Engineering for Advanced Materials (SEAM), School of Science, Swinburne University of Technology, Hawthorn, VIC 3122, Australia, Australia
2. Optical Sciences Centre and ARC Training Centre in Surface Engineering for Advanced Materials (SEAM), School of Science, Swinburne University of Technology, Hawthorn, VIC 3122, Australia
3. Institute of Physics, University of Tartu, W. Ostwaldi 1, 50411 Tartu, Estonia
4. Infrared Microspectroscopy (IRM) Beamline, ANSTO—Australian Synchrotron, Clayton, VIC 3168
5. Tokyo Tech World Research Hub Initiative, School of Materials and Chemical Technology, Tokyo Institute of Technology, 2-12-1, Ookayama, Meguro-ku, Tokyo 152-8550, Japan
Abstract

Near-IR (NIR) region of the synchrotron-IR beam, which is usually filtered out in order to improve the signal to noise ratio of Fourier-transform infrared spectroscopy (FTIR), was extracted and used as an illuminating source to achieve phase imaging of biochemical samples. A 200-um pinhole was aligned with one lobe of the unique fork shaped NIR synchrotron beam at a resulting intensity maximum. The diffracted light with airy diffraction pattern passed the biochemical sample and was collected by NIR sensitive lensless camera. The Gerchberg-Saxton algorithm (GSA) was used to reconstruct the phase images of the samples from recorded the intensity images.

Keywords
phase imaging
bioimaging
synchrotron
near infrared beam
holography
incoherent optics
chemical imaging
phase retrieval
3D imaging
Manuscript
Oral Presentation
Poster
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