Events MOL2NET'23, Conference on Molecular, Biomed., Comput. & Network Science and Engineering, 9th ed.

Event submissions

Published

10.3390/mol2net-09-14328 (registering DOI)

This submission belongs to the session 02. CHEMBIO.MOL-09: Org. Chem., Med. Chem., Mol. Biol., & Pharm. Industry Congress, Paris, France-Fargo, USA, 2023. of the event MOL2NET'23, Conference on Molecular, Biomed., Comput. & Network Science and Engineering, 9th ed.

Published date

03 Apr, 2023

Academic Editor

Humbert G. Díaz

Citation

Beniamin Oskar Grabarek, Michał Czerwiński, Emilia Morawiec, Dariusz Boroń, Marcin Oplawski, Variances in the Expression of mRNAs Related to the Histaminergic System in Endometrioid Endometrial Cancer, in Proceedings of MOL2NET'23, Conference on Molecular, Biomed., Comput. & Network Science and Engineering, 9th ed., 25 December–31 December 2023, MDPI: Basel, Switzerland, doi: 10.3390/mol2net-09-14328

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Variances in the Expression of mRNAs Related to the Histaminergic System in Endometrioid Endometrial Cancer

Michał Czerwiński ¹

Emilia Morawiec ²

Dariusz Boroń ³

Marcin Oplawski ⁴

Beniamin Oskar Grabarek ^5,6

1. Department of Histology, Cytophysiology and Embryology, Faculty of Medicine, Academy of Silesia, 41-800 Zabrze, Poland

2. Department of Histology, Cytophysiology and Embryology, Faculty of Medicine in Zabrze, Academy of Silesia, 40-055 Katowice, Poland

3. Department of Histology, Cytophysiology and Embryology, Faculty of Medicine, University of Technology in Katowice, 41-800 Zabrze, Poland

4. Department of Gynecology and Obstetrics with Gynecologic Oncology, Ludwik Rydygier Memorial Specialized Hospital, 31-826 Kraków, Poland

5. Department of Histology, Cytophysiology and Embryology, Faculty of Medicine in Zabrze, Academy of Silesia, 40-555 Katowice, Poland, Poland

6. Department of Neurosurgery, 5th Military Clinical Hospital with the SP ZOZ Polyclinic in Krakow, 30-901 Krakow, Poland

Abstract

Research has indicated higher concentrations of histamine and polyamine in endometrioid tissue in comparison with healthy tissue. The aim of this study was to evaluate changes in the expression patterns of messenger RNA (mRNAs) and microRNA (miRNAs) related to the histaminergic system in endometrial samples and whole blood in women with endometrioid endometrial cancer. The study group consisted of 30 women with endometrioid endometrial cancer qualified for hysterectomy (G1 well-differentiated, 15 cases; G2 moderately differentiated, 8 cases; and G3 poorly differentiated, 7 cases). The control group included 30 women with no neoplastic changes during routine gynecological examinations. The molecular analysis consisted of the microarray analysis of mRNAs and miRNAs related to the histaminergic system, reverse-transcription quantitative polymerase chain reaction (RTqPCR), and enzyme-linked immunosorbent assay (ELISA). Out of 65 mRNAs connected with the histaminergic system, 10 differentiate the samples of tissue and blood obtained from patients with endometrioid endometrial cancer in comparison with the control group (p < 0.05). mRNA histamine receptor 1,3 (HRH1, HRH3), and solute carrier family 22 member 3 (SLC23A2) differentiating samples of endometrioid endometrial cancer independent of either G or control. The selected mRNA transcripts seem to be promising for molecularly targeted therapies in the context of endometrioid endometrial cancer.

Keywords

endometrioid endometrial cancer

grading

histaminergic system

molecular marker

Manuscript

michal czerwinski.pdf

Oral Presentation

pawel m.pptx

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