Formulation of modified microspheres based on galactosylated lactic acid polymers

A new series of galactosyl-derived polymers has been used for the preparation of microspheres. The strategy is based on the modification of the terminal carboxylic group L-PLA by coupling to a galactosyl antenna in the presence of the peptide coupling agents : DCC/HOBT . the degree of functionalisation varies between 60 and 70%, and antenna density between 1.74 and 2.78. In an effort to develop a new way of drug delivery, especially for polymeric antifungal molecules, we have incorporated amphotericin B (AmB) into biodegradable galactosylated poly(L-lactic acid) L-PLA . These drug carriers were prepared by solvent evaporation method using an oil/water (o/w) emulsion. The ratio of galactosylated microspheres was 7.14 mg for L-PLA (encapsulation rate 45% of mole). In our yeast model, drug release depend on three factors : i) presence of galactosylated antennae, ii) length of galactosyl antenna and iii) nature of the polymer . These novel functionalized microspheres could be required for the delivering of therapeutic agents according to their recognition to specific cells .