Events The 3rd International Electronic Conference on Biosensors

Event submissions

Published

10.3390/IECB2023-14599

This submission belongs to the session E. Microfabrication and Printing Techniques in Biosensors of the event The 3rd International Electronic Conference on Biosensors

Published date

12 May, 2023

Academic Editor

Giovanna Marrazza

Citation

Samar Damiati, Shakil A. Awan, Martin Peacock, Bernhard Schuster, Functionalization of Graphene Oxide for Label-free Electrochemical Detection of Hepatic Cancer Cells, in Proceedings of The 3rd International Electronic Conference on Biosensors, 8 May–21 May 2023, MDPI: Basel, Switzerland, doi: 10.3390/IECB2023-14599

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Functionalization of Graphene Oxide for Label-free Electrochemical Detection of Hepatic Cancer Cells

Samar Damiati ¹

Shakil A. Awan ²

Martin Peacock ³

Bernhard Schuster ⁴

1. Department of Chemistry, College of Sciences, University of Sharjah, Sharjah, UAE, United Arab Emirates

2. Wolfson Nanomaterials and Devices Laboratory, School of Engineering, Computing and Mathematics, Faculty of Science and Engineering, University of Plymouth, Plymouth, UK

3. Zimmer and Peacock Ltd., Royston SG8 9JL, UK

4. Institute of Synthetic Bioarchitecture, Department of Bionanosciences, University of Natural Resources and Life Sciences, 1190 Vienna, Austria

Abstract

In this study, a graphene oxide (GO) modified screen-printed carbon electrode (SPE) was functionalized with 1-pyrenebutyric acid-N-hydroxy-succinimide ester (PANHS) and conjugated with antibodies for the label-free detection of human hepatoma HepG2 cells (Fig. 1). Using a user-friendly reservoir chamber, the functionalized film was exposed continuously to the cancer cells. The continuous flow is intended to enhance capture of the target cells onto the sensing platform. The response of the biosensor was evaluated using cyclic voltammetry (CV). The preliminary data showed good sensitivity for detection of hepatic cancer cells. The developed biosensor could detect the HepG2 cells from 1 × 10³ to 3 × 10⁵ cells/mL range. Thus, it provides a simple tool for electrochemical detection of cancer cells and offers low-cost and disposable sensing platform.

Figure 1. Schematic illustration of the developed electrochemical biosensor for detection of cancer cells (not drawn to scale).

Keywords

Electrochemical biosensor

Flow cell

Graphene Oxide

Linker

Manuscript

sciforum-071110 done.pdf

Molecularly Imprinted Polymers-coated Fluorescent Nanomaterials for Detection of Antibiotic Residues

Electrochemistry of freely diffusing mediators in polyelectrolyte membranes used for blood glucose test-strips with a high upper limit of the linear range.