EventsThe 2nd International Electronic Conference on Chemical Sensors and Analytical Chemistry
Event submissions
Published
with-doi10.3390/CSAC2023-14913
This submission belongs to the session S1. Electrochemical Devices and Sensors of the event The 2nd International Electronic Conference on Chemical Sensors and Analytical Chemistry
Published date
26 Sep, 2023
Academic Editor
author-avatarNicole Jaffrezic-Renault
Citation
M. Fátima Barroso, Renato Caldevilla, Stephanie L. Morais, Serafim Carvalho, Rui Medeiros, Cristina Delerue-Matos, Agostinho Cruz, Marlene Santos, Design of an electrochemical genosensor for the BDNF gene polymorphism sequence detection using an enzymatic labelled DNA probe, in Proceedings of The 2nd International Electronic Conference on Chemical Sensors and Analytical Chemistry, 16 September–30 September 2023, MDPI: Basel, Switzerland, doi: 10.3390/CSAC2023-14913
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Design of an electrochemical genosensor for the BDNF gene polymorphism sequence detection using an enzymatic labelled DNA probe

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Renato Caldevilla 1,2
Stephanie L. Morais 2
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Serafim Carvalho 3,4
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Rui Medeiros 5,6
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Cristina Delerue-Matos 2
Agostinho Cruz 1
Marlene Santos 1,5
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M. Fátima Barroso 2
1. CISA|ESS, Centro de Investigação em Saúde e Ambiente, Escola Superior de Saúde, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida, 400, 4200-072, Porto, Portugal
2. REQUIMTE/LAQV, Instituto Superior de Engenharia do Porto, Instituto Politécnico, Rua Dr. António Bernardino de Almeida 431, 4200-072, Porto, Portugal
3. Hospital de Magalhães Lemos, 4149-003 Porto, Portugal
4. Instituto Universitário de Ciências da Saúde, 4585-116 Gandra, Portugal
5. Molecular Oncology & Viral Pathology, IPO-Porto Research Center (CI-IPOP), Portuguese Institute of Oncology, 4200-072 Porto, Portugal
6. Research Department, Portuguese League Against Cancer (Norte), 4200-172 Porto, Portugal
Abstract

The BDNF gene is associated with high degrees of variability in antidepressant treatments. The Val66Met polymorphism is widely known as a source of this variability, warranting growing interest in genotyping patients that undergo antidepressant treatment to better suit their needs. This paper reports on an electrochemical genosensing platform, based on gold electrodes, capable of detecting this polymorphism, through the use of synthetic enzymatic labelled DNA-probes for 2 different BDNF alleles. The sensor showed promising results, and its applicability to real samples is currently being tested.

Keywords
Chronoamperometry
Depression
Electrochemical genosensor
Manuscript
Design of an electrochemical genosensor for the BDNF gene polymorphism sequence detection using an enzymatic labelled DNA probe- Renato Caldevilla.pdf
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