THE INFLUENCE OF WHEAT-FLOUR ENRICHED WITH WILD GROWN FRUITS ON THE PROLIFERATION OF FIBROBLASTS, BREAST CANCER CELLS, AND MELANOMA CELLS

The number of deaths due to malignant neoplasms is increasing year by year. For this reason, new ways of preventing them and supporting treatment are being sought. One of them is adding plant extracts to food in case of increasing its antioxidant, anti-inflammatory and anti-cancerogenic activity. The aim of the study was to examine the effect of different wild grown fruits (chokeberry, elderberry, hawthorn and sea-buckthorn) added to wheat-flour cookies on the proliferation of: (i) normal BJ lines (fibroblasts); (ii) tumour cells of the MCF-7 (breast cancer) and (iii) WM793 (melanoma) lines. Methanol-acetone extracts were prepared from previously baked wheat-flour cookies fortified with fruits, in order to use them in the further part of the research to prepare mixtures with concentrations of: 0.5 mg / mL; 1 mg / mL; 1.5 mg / mL; 2.5 mg / mL. The viability and cytotoxicity of normal and neoplastic cells was examined. It was observed that the MCF-7 melanoma tumour line appeared to be more susceptible to the action of the tested extracts with the addition of selected wild grown fruits compared to WM793 breast cancer cells. Moreover, the greatest significant effect on the inhibition of MCF-7 cells among extracts with a concentration of 2.5 mg/ml was proved in the case of chokeberry and elderberry (p <0.05). In terms of the inhibition of the WM793 line among extracts with a concentration of 2.5 mg/ml, the greatest significant effect was proved in the case of sea buckthorn and elderberry (p <0.05).