Algal Organic Matter Fluorescence Analysis of Chlorella sp. for Biomass Estimation

Algal Organic Matter (AOM) is derived from dissolved organic matter composition of the algal species observed. In this study, excitation-emission fluorescence spectroscopy has been used to determine the AOM and pigment characteristics of Chlorella sp. in varying algal biomass concentrations. The AOM and pigment characteristics were observed at 400-600 nm and 600-800 nm fluorescence emission, respectively with excitation spectrum of 300-450 nm. F680/450 is computed based on the ratio between the chlorophyll-a at 680 nm and dissolve organic matter contribution at 450 nm. F680/450 showed positive correlation with algal biomass (R² = 94.45), estimated chlorophyll-a pigment (R² = 87.12) of Chlorella sp. This study is a good reference for algal growth monitoring in biomass estimation and production in natural waters.