EventsThe 3rd International Electronic Conference on Antibiotics
Event submissions
Published
with-doi10.3390/ECA2023-16399
This submission belongs to the session S3. New Antimicrobial Approaches, Targets and Mechanisms of Action of the event The 3rd International Electronic Conference on Antibiotics
Published date
30 Nov, 2023
Academic Editor
author-avatarMarc Maresca
Citation
Vanessa Silva, Jessica Ribeiro, Rupesh Kumar Singh, Alfredo Aires, Rosa Carvalho, Virgílio Falco, José Eduardo Pereira, Gilberto Igrejas, Patrícia Poeta, Exploring Winemaking By-Products of Tinto Cão Grapes: Antioxidant and Antimicrobial Activity Against Multiresistant Bacteria, in Proceedings of The 3rd International Electronic Conference on Antibiotics, 1 December–15 December 2023, MDPI: Basel, Switzerland, doi: 10.3390/ECA2023-16399
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Exploring Winemaking By-Products of Tinto Cão Grapes: Antioxidant and Antimicrobial Activity Against Multiresistant Bacteria

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Vanessa Silva 1
Jessica Ribeiro 2,3,4
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Rupesh Kumar Singh 5
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Alfredo Aires 6
Rosa Carvalho 7
Virgílio Falco 5
José Eduardo Pereira 8,9,10
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Gilberto Igrejas 3,4,11
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Patrícia Poeta 10,12,13
1. Associated Laboratory for Green Chemistry (LAQV-REQUIMTE), University NOVA of Lisboa, Lisboa, Caparica, Portugal, Portugal
2. Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal, Portugal
3. Department of Genetics and Biotechnology, Functional Genomics and Proteomics’ Unit, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
4. Associated Laboratory for Green Chemistry (LAQV-REQUIMTE), University NOVA of Lisboa, Lisboa, Caparica, Portugal
5. Chemistry Research Centre (CQ-VR), University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal
6. Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal
7. Department of Agronomy, School of Agrarian and Veterinary Sciences, University of Trás‐os‐Montes e Alto Douro (UTAD), Vila Real, Portugal
8. Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal
9. Veterinary and Animal Research Centre, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal
10. Associate Laboratory for Animal and Veterinary Science (AL4AnimalS), University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal
11. Functional Genomics and Proteomics Unit, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal
12. Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal
13. CECAV—Veterinary and Animal Research Centre, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal
Abstract

The escalating global concern over antimicrobial resistance has necessitated the search for alternative strategies to combat multidrug-resistant bacterial infections. Natural compounds, particularly those derived from plant sources, have emerged as promising alternatives to antibiotics. In this context, this study aimed to explore the antimicrobial potential of phenolic compounds extracted from winemaking by-products of Tinto Cão grapes against 10 multidrug-resistant bacterial strains. Additionally, the antioxidant activity of these compounds was evaluated.

Skin, seed, stem, shoat, and leaf sub-products from Tinto Cão grapes were extracted for their phenolic compounds. The antimicrobial activity was assessed against a panel of multidrug-resistant bacteria, including S. epidermidis, S. aureus, L. monocytogenes, E. faecium, E. faecalis, B. cereus, K. pneumoniae, P. aeruginosa, S. enteritidis and E. coli using MIC values. The antioxidant potential was measured using EC50 values obtained from DPPH, FRAP, and CuPRAC assays.

Among the bacterial strains tested, S. epidermidis, S. aureus, K. pneumoniae, P. aeruginosa, B. ceureus, and L. monocytogenes displayed susceptibility to the extracted phenolic compounds. The shoat extracts showed the highest antimicrobial activity, inhibiting six out of the ten bacterial strains and displaying lower MIC values ranging from 10 to 50 mg/mL. The lowest MIC value obtained was with the shoat extract against S. aureus, with a MIC of 10 mg/mL. Seed extracts demonstrated the highest antioxidant activity with the DPPH and CuPRAC methods (0.63±0.02 and 0.51±0.002, respectively), while leaf extracts exhibited the highest antioxidant activity with the FRAP method (0.55±0.001).

Our study showcases potent antimicrobial activity of phenolic compounds from Tinto Cão grape winemaking by-products against multidrug-resistant bacteria. These findings suggest their potential as a natural solution to combat antimicrobial resistance, promoting sustainable and effective strategies beyond traditional antibiotics.

Keywords
antimicrobial resistance
antioxidant activity
wine by-products
polyphenols
bioactive compounds
Manuscript
sciforum-077573-clean.pdf
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